文献类型：期刊文献

英文题名：The bio-functional role of calcium in mesoporous silica xerogels on the responses of osteoblasts in vitro

作者：Zhou, Huanjun[1];Wei, Jie[1];Wu, Xiaohui[1];Shi, Jianlin[1];Liu, Changsheng[1];Jia, Junfeng[1];Dai, Chenglong[1];Gan, Qi[1]

机构：[1]E China Univ Sci & Technol, Ctr Biomed Mat, Key Lab Ultrafine Mat Minist Educ & Engn, Minist Educ, Shanghai 200237, Peoples R China

年份：2010

卷号：21

期号：7

起止页码：2175

外文期刊名：JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

收录：;EI(收录号：20102813075721);WOS:【SCI-EXPANDED(收录号:WOS:000279592600019)】；

基金：The authors are indebted to the financial support from the National Natural Science Foundation of China (No. 30670568, 50732002), Program of Shanghai Subject Chief Scientist (No. 07XD14008), and Shanghai Nanotechnology Special Foundation (No. 0852nm02700), Major Program of National Natural Science Foundation of China (No. 50732002), Program for Changjiang Scholars and Innovative Research Team in University.

语种：英文

外文关键词：Silica gel - Gene expression - Sol-gels - Cell proliferation - Sol-gel process - Xerogels - Biocompatibility - Osteoblasts - Titanium - Controlled drug delivery - Activated carbon - Mesoporous materials

摘要：Mesoporous silica xerogels with various amount of calcium (0, 5, 10 and 15%, named m-SXC0, m-SXC5, m-SXC10 and m-SXC15, respectively) were synthesized by template sol-gel methods, and cell responses to m-SXCs were studied using murine pre-osteoblast MC3T3-E1 in vitro. The results showed that cell morphology was not affected by m-SXCs indicating good biocompatibility. Furthermore, cell proliferation ratio on the m-SXCs increased over time, among which m-SXC10 was highest. NO production obviously rose with the increase of Ca content in m-SXCs. ALP activity and PGE(2) level on m-SXC5 significantly improved compared with m-SXC0 while decreased with the increase of Ca content for m-SXC10 and m-SXC15. No obvious discrepancy on osteopontin mRNA expressions was observed among m-SXCs. The collagen I and osteocalcin mRNA expression on m-SXC5 were up-regulated, while decreased on m-SXC15 evidently. The phosphorylation level of ERK 1/2 for the m-SXC10 was highest after 7 days. In conclusion, calcium in m-SXCs plays an important role in osteoblast activity, which indicates mesoporous silica xerogel containing appropriate calcium could stimulate osteoblast proliferation, differentiation, gene expression via the activation of ERK 1/2 signaling pathway, and shows great prospects in bone regeneration field using as a drug controlled release filler.