文献类型：期刊文献

英文题名：Imaging fibrinogen assembly on calcium-doped titanium by using scanning electrochemical microscopy

作者：Liu, Pengyu[1];Li, Liang[2];Cao, Huiliang[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Mat Sci & Engn, Interfacial Electrochem & Biomat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2024

卷号：202

外文期刊名：MICROCHEMICAL JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001243610800001)】；

基金：This work was jointly supported by grants from the National Natural Science Foundation of China (32271399 and 31870945) , the Natural Science Foundation of Shanghai (21ZR1415700) , and the Shanghai Committee of Science and Technology (23S31901700) . The authors gratefully acknowledge Mr. Zhong Wang's contribution during the revision stage.

语种：英文

外文关键词：Protein adsorption; Scanning electrochemical microscopy; Dye -assist scanning electron microscopy; Surface modification; Ion implantation

摘要：Fibrinogen adsorption is a critical host response to the insertions of various medical devices. Scanning electrochemical microscopy (SECM) has the power to probe the local interactions between proteins and bulk solids in liquid environments; however, our knowledge of the distribution or the assembly structures of protein adsorption is limited. Here, fibrinogen assembly behaviors on calcium-doped titanium are imaged by resolving the strength of the SECM probe's negative feedback in two-dimensional micro-regions. By using ion implantation techniques, calcium and/or silver were doped into polished titanium surfaces. The calcium-doped titanium was capable of releasing calcium, while the clean titanium and silver-doped titanium did not release their constituents. The twodimensional discontinuous distribution of the probe current/steady current (i/i0) values were only detectable in calcium-doped groups, and this was incubating duration dependent, showing the micro-regions preferential for fibrinogen assembly in calcium-doped groups. This was consistent with the fibrinogen adsorption morphologies on calcium-doped titanium examined by dye-assisted scanning electron microscopy. It is concluded that protein assembly behaviors on bulk solid surfaces in liquid environments can be imaged by resolving the distribution of the SECM probe currents in two-dimensional areas. The method is promising in illuminating the true surfaceprotein interactions and the protein adsorption structure-function relations.