文献类型：期刊文献

中文题名：NCL-based mitochondrial-targeting fluorescent probe for the detection of Glutathione in living cells

作者：Tongxia Jin[1];Mengyu Cui[1];Dan Wu[1];Weiping Zhu[1];Yufang Xu[1];Xuhong Qian[1]

机构：[1]State Key Laboratory of Bioreactor Engineering,Shanghai Key Laboratory of Chemical Biology,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China

年份：2021

卷号：32

期号：12

起止页码：3899

中文期刊名：Chinese Chemical Letters

外文期刊名：中国化学快报（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2021_2022】；PubMed;

基金：the National Natural Science Foundation of China (Nos. 21878088, 21476077);Key Project of the Shanghai Science and Technology Committee (No. 18DZ1112703) for financial support。

语种：英文

中文关键词：Fluorescent probe;Glutathione;NCL;In vivo imaging

摘要：Glutathione(GSH) plays a critical role in maintaining cellular redox homeostasis in biological system.Mitochondrion is a pivotal organelle for cellular aerobic respiration and its disorder is associated with impaired redox balance, leading to cell death. In this work, we designed and synthesized a non-invasive“off-on” mitochondrial-targeting fluorescent probe QZ for the detection of GSH in living cells. Based on the mechanism of native chemical ligation(NCL) and fluorescence resonance energy transfer(FRET), a rhodamine B derivative, QZ was prepared, by choosing aromatic thioester bond as the selective reaction site. QZ exhibited excellent detection capability for GSH over Cys and Hcy. Upon addition of GSH to QZ solution, a remarkably enhanced fluorescence was observed with a limit of detection of 2.98 μmol/L.Furthermore, QZ was found to possess the specific mitochondrial localization ability in cell imaging experiments. Moreover, with exogenous and endogenous stimulations, QZ could image GSH in living cells.