文献类型：期刊文献

中文题名：表面接枝透明质酸聚氨酯的合成及其血液相容性

英文题名：Synthesis and Haemocompatibility Evaluation of Hyaluronan Immobilized Polyurethane

作者：郭会[1];程树军[1];龚飞荣[1];杨丽静[1]

机构：[1]华东理工大学材料科学与工程学院,超细材料制备与应用教育部重点实验室,上海200237

年份：2010

卷号：36

期号：4

起止页码：512

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家863项目(2007AA02Z450)

语种：中文

中文关键词：聚氨酯;表面接枝;血液相容性;生物相容性

外文关键词：polyurethane; surface immobilization; haemocompatibility; biocompatibility

摘要：通过两步溶液法合成了侧链带有羧基的聚氨酯(PU),成膜后其表面羧基经1-乙基-3-(二甲基丙胺)碳二亚胺(EDC)活化后与1,3-丙二胺反应,合成了氨基化聚氨酯材料。透明质酸(HA)经EDC活化后共价接枝到氨基化的聚氨酯膜表面。通过衰减全反射光谱法(ATR-FTIR),静态水接触角测试和扫描电镜(SEM)对聚氨酯接枝前后的结构和形态进行表征,并通过凝血实验和内皮细胞培养实验,测试材料的抗凝血性和细胞毒性。结果表明:透明质酸成功地接枝到聚氨酯膜表面,接枝透明质酸后的聚氨酯膜亲水性提高,凝血时间大幅延长,且能促进内皮细胞的黏附和生长,体现较好的血液相容性。
Polyurethanes containing carboxyl side groups were polymerization. Hyaturonan （HA） was then immobilized onto synthesized by a two-step solution the surface of amino-functionalized polyurethane film which was prepared by reacting with 1,3-diaminopropane in the presence of 1-ethyl-3-E3- （dimethylaminopropyl）] carbodiimide hydrochlorid （EDC）. Attenuated total reflection Fourier transform infrared spectroscopy （ATR-FTIR）, water contact angle measurement and scanning electron microscopy （SEM） were used to confirm the surface ehanges at each step of treatment, both in morphology and chemical composition. The anticoagulation activity and cytotoxicity of the polymers were also studied. The results showed that hydrophilicity and blood compatibility of the HA immobilized polyurethane were improved. Cytotoxieity analysis indicated that the modified polyurethane can support human vein endothelial cells （HUVECs） adhesion and proliferation. The novel material with potent antithrombogenic properties could be used in blood-contacting applications.