文献类型：期刊文献

英文题名：Enhancing aspergiolide A production from a shear-sensitive and easy-foaming marine-derived filamentous fungus Aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor

作者：Cai, Menghao[1];Zhou, Xiangshan[1];Lu, Jian[1];Fan, Weimin[1];Niu, Chuanpeng[1];Zhou, Jiushun[1];Sun, Xueqian[1];Kang, Li[1];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2011

卷号：102

期号：3

起止页码：3584

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000286904500209)】；

基金：This work was supported by the National High Technology Research and Development Program of China (2010AA09Z429), Shanghai Rising-Star Program (09QA1401600) and Shanghai Leading Academic Discipline Project (B505). We thank Ocean University of China for supply of the strain and the aspergiolide A standard.

语种：英文

外文关键词：Aspergillus glaucus; Aspergiolide A; Oxygen carrier; Shear-sensitive

摘要：Production enhancement of a novel antitumor compound aspergiolide A from shear-sensitive and easy-foaming marine-derived fungus Aspergillus glaucus HB1-19 in a 5-l stirred bioreactor was investigated. Two types of impellers. i.e., six-flat-blade disc turbine impeller (DT) and three-sector-blade pitched blade turbine impeller (PB) were used in this work. In cultures with fermentation medium, the combination of upper PB and lower DT led to the maximum dry biomass (13.8 g/l) and aspergiolide A production (19.3 mg/l). However, two PBs brought the highest aspergiolide A yield coefficient (1.9 mg/g dry biomass) despite it produced the lowest dry biomass (5.3 g/l). By contrast, two DTs and the upper DT and lower PB showed insignificant results. Feeding 0.35% (v/v) n-dodecane in cultures with upper PB and lower DT further improved aspergiolide A production by 31.0%, i.e., 25.3 mg/l, which is also 322% higher than that in the ordinary cultures with two DTs. (C) 2010 Elsevier Ltd. All rights reserved.