文献类型：期刊文献

英文题名：A reduced polydopamine nanoparticle-coupled sprayable PEG hydrogel adhesive with anti-infection activity for rapid wound sealing

作者：Sun, Junjie[1];Tan, Haoqi[1];Liu, Huan[1];Jin, Dawei[2];Yin, Meng[2];Lin, Haodong[3];Qu, Xue[1];Liu, Changsheng[1]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Mat Sci & Engn, Key Lab Ultrafine Mat,Minist Educ, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Shanghai Childrens Med Ctr, Sch Med, Dept Cardiothorac Surg, 1678 Dong Fang Rd, Shanghai 200127, Peoples R China;[3]Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Orthoped Surg, Sch Med, Shanghai 200080, Peoples R China

年份：2020

卷号：8

期号：24

起止页码：6946

外文期刊名：BIOMATERIALS SCIENCE

收录：;EI(收录号：20205209679216);WOS:【SCI-EXPANDED(收录号:WOS:000600664500013)】；

基金：The support from the National Natural Science Foundation of China (31922041, 51621002, and 11932012), the Science and Technology Innovation Project of Shanghai Science and Technology Committee (18441908300), and the Fundamental Research Funds for the Central Universities (SLD 00202006, JKVD12001032, JKVD12001002) is acknowledged.

语种：英文

外文关键词：Gelation - Infrared devices - Adhesives - Hydrogels - Nanoparticles - Redox reactions

摘要：There is a growing demand to develop sprayable hydrogel adhesives with rapid-forming and antibacterial abilities to instantly seal open wounds and combat pathogen infection. Herein, we propose to design a polydopamine nanoparticle (PDA NP) coupled PEG hydrogel that can quickly solidify via an amidation reaction after spraying as well as tightly binding PDA NPs to deliver reactive oxygen species (ROS) and induce a photothermal effect for bactericidal activity, and provide a hydrophilic surface for antifouling activity. The molecular structure of the 4-arm-PEG-NHS precursor was regulated to increase its reactivity with 4-arm-PEG-NH2, which thus shortened the gelation time of the PEG adhesive to 1 s to allow a fast solidification after being sprayed. The PEG-NHS precursor also provided covalent binding with tissue and PDA NPs. The reduced PDA NPs have redox activity to convey electrons to oxygen to generate ROS (H2O2), thus endowing the hydrogel with ROS dependent antibacterial ability. Moreover, NIR irradiation can accelerate the ROS release because of the photothermal effect of PDA NPs. In vitro tests demonstrated that H2O2 and the NIR-photothermal effect synergistically induced a fast bacterial killing, and an in vivo anti-infection test also proved the effectiveness of PEG-PDA. The sprayable PEG-PDA hydrogel adhesive, with rapid-forming performance and a dual bactericidal mechanism, may be promising for sealing large-scale and acute wound sites or invisible bleeding sites, and protect them from pathogen infection.