文献类型：期刊文献

英文题名：A ratiometric near-infrared fluorescence strategy based on spiropyran in situ switching for tracking dynamic changes of live-cell lysosomal pH

作者：Li, Jin[1];Li, Xiaokang[2];Jia, Jianbo[1];Chen, Xi[1];Lv, Yinjuan[1];Guo, Yuan[1];Li, Jian[2]

机构：[1]Northwest Univ, Coll Chem & Mat Sci, Natl Demonstrat Ctr Expt Chem Educ, Key Lab Synthet & Nat Funct Mol Chem,Minist Educ, Xian 710127, Shaanxi, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：166

起止页码：433

外文期刊名：DYES AND PIGMENTS

收录：;EI(收录号：20191406732356);WOS:【SCI-EXPANDED(收录号:WOS:000466823300054)】；

基金：This work was supported by the National Key R&D Program of China (2017YFB0202600), the National Natural Science Foundation of China (21472148, 21072158 and 21807088), the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204), Open Funding Project of the State Key Laboratory of Bioreactor Engineering (20180PEN12), Overseas Students Science and Technology Activities Project Merit Funding of Shaanxi Province (20151190) and Academic Backbone of Northwest University Outstanding Youth Support Program.

语种：英文

外文关键词：Ratiometric sensing; NIR fluorescent probe; Spiropyran; Fluorescence imaging; Lysosomal pH

摘要：Abnormal lysosomal pH values have been associated with cellular dysfunctions and many diseases. However, tools to monitor dynamic changes of pH values in live-cell lysosomes are limited. Herein, biocompatible near-infrared (NIR) fluorescent dyes HAN1-4 for lysosome-targeting staining were rationally designed and synthesized with the aid of a spiropyran in situ switching (SIS) strategy. The novel SIS-based dyes share the following special characteristics: 1) pH-dependent ratiometric fluorescent patterns matched nicely with pH windows of lysosomes, 2) light-independent real-time spiropyran in situ switching behavior in lysosomes and 3) pH-sensitive fluorescence in the NIR region, thereby ensuring their applications in obtaining highly specific images of live-cell lysosomes with a weak background signal. Among them, the HAN2 probe was found to induce the best ratio-metric response toward pH in the range corresponding to lysosomal pH window, thus being further applied for the real-time tracking of lysosomal subtle pH changes in stressed cells undergoing drug and heat stimulations. Live-cell super-resolution and confocal fluorescent imaging indicate that our SIS-based NIR probes are robust and can be applied for the accurate detection of lysosomal pH in complex biosystems.