文献类型：期刊文献

英文题名：Ratiometric and light-up near-infrared fluorescent DCM-based probe for real-time monitoring endogenous tyrosinase activity

作者：Li, Qiang[1];Yan, Chenxu[1];Zhang, Jie[1];Guo, Zhiqian[1];Zhu, Wei-Hong[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Joint Int Res Lab Precis Chem & Mol Engn,Sch Chem, Key Lab Adv Mat & Inst Fine Chem,Feringa Nobel Pr, Shanghai 200237, Peoples R China

年份：2019

卷号：162

起止页码：802

外文期刊名：DYES AND PIGMENTS

收录：;EI(收录号：20184606082714);WOS:【SCI-EXPANDED(收录号:WOS:000456226500094)】；

基金：This work is supported by NSFC/China (21788102, 21421004, 21636002 and 21622602), National key Research and Development Program ((2017YFC0906902 and 2016YFA0200300), Oriental Scholarship, Scientific Committee of Shanghai (14ZR1409700 and 15XD1501400), Shanghai Pujiang Program (13PJD010), Fok Ying Tong Education Foundation (142014), the Fundamental Research Funds for the Central Universities (WK1013002), and Programme of Introducing Talents of Discipline to Universities (B16017).

语种：英文

外文关键词：Fluorescence; Ratiometric probe; Tyrosinase; Near-infrared; Dicyanomethylene-4H-pyran

摘要：Tyrosinase (TYR), which is regarded as a significant biomarker of melanoma cancer, plays a crucial but incompletely understood role in cellular biochemistry and etiology. In this work, we report a TYR activatable NIR fluorescent probe DCM-1, which is composed of dicyanomethylene-4H-pyran (DCM) unit as the NIR fluorescence reporter, amide bond specific for TYR cleavage, and phenol group as the triggered moiety. The activation of DCM-1 by endogenous TYR can induce a remarkable fluorescence red shift from 548 to 660 nm, allowing for ratiometric and light-up NIR response to real-time track TYR activity. Besides, DCM-1 exhibits extraordinary features, such as excellent sensitivity, high selectivity, and high photostability. In vitro experiments by confocal laser scanning microscopy reveals that the spatial-temporal information of TYR in B16 melanoma cancer cells can be achieved by the probe DCM-1. Thus, this enzyme-activatable NIR probe can be a powerful tool for elucidating the roles of TYR in biological systems.