文献类型：期刊文献

英文题名：Analysis of the inhibition of nucleic acid dyes on polymerase chain reaction by capillary electrophoresis

作者：Li, Zhenqing[1];Liu, Chenchen[1];Ma, Siyao[1];Zhang, Dawei[1];Yamaguchi, Yoshinori[2,3]

机构：[1]Univ Shanghai Sci & Technol, Engn Res Ctr Opt Instrument & Syst, 516 Jungong Rd, Shanghai 200093, Peoples R China;[2]ECUST, IPBM, Grad Sch Sci, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Osaka Univ, Dept Appl Phys, Grad Sch Engn, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan

年份：2016

卷号：8

期号：11

起止页码：2330

外文期刊名：ANALYTICAL METHODS

收录：;EI(收录号：20161202128664);WOS:【SCI-EXPANDED(收录号:WOS:000372255100002)】；

基金：We gratefully acknowledge support from the National Natural Science Foundation of China (No. 21205078) and Research Fund for the Doctoral Program of Higher Education of China (No. 20123120110002), and this project was partly supported by Grand-in-Aid for Scientic Research (Houga) (No. 25600049 (Y. Y.)), JSPS, Japan. We also thank Mr Tsuji at Yamato Esulon Co., Ltd (Osaka, Japan) for the technical assistance in this experiment.

语种：英文

外文关键词：Capillary electrophoresis - Chains - Diagnosis

摘要：An integrated polymerase chain reaction (PCR) and capillary electrophoresis (CE) system can realize accurate quantification of the target PCR product by adding labeling dyes to the PCR reagents, because CE can discriminate all the subsequent nucleic acids, including the primers, non-specific and specific PCR products. Here we discuss the inhibition of labeling dyes on PCR by performing the PCR of Porphyromonas gingivalis (PG) with solutions containing Hoechst 33258, SYBR Green I, and SYBR Green II. Results demonstrated that Hoechst 33258 totally inhibited the PCR process, and PCR efficiency was highly dependent on the concentration of SYBR Green I/II. Such a study expands the capabilities of CE and contributes greatly to the development of hyphenated PCR-CE instruments for biological and medical diagnosis.