文献类型：期刊文献

英文题名：Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30

作者：Zhang, Liaoyuan[1]; Yang, Yunlong[1]; Sun, Jian'an[1]; Shen, Yaling[1]; Wei, Dongzhi[1]; Zhu, Jiawen[2]; Chu, Ju[3]

机构：[1] State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China; [2] Chemical Engineering Research Center, East China University of Science and Technology, Shanghai 200237, China; [3] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

年份：2010

卷号：101

期号：6

起止页码：1961

外文期刊名：Bioresource Technology

收录：EI(收录号：20104413345626)

语种：英文

外文关键词：Sodium compounds - Fermentation - Sugar (sucrose)

摘要：The production of 2,3-butanediol (2,3-BD) by Serratia marcescens H30 from sucrose was studied. Medium composition for 2,3-BD production by S. marcescens H30 was optimized in shake flask fermentations using Plackett-Burman design (PB) and response surface methodology (RSM). Results indicated that yeast extract and sodium acetate had significant effects on the 2,3-BD production. And their optimal concentrations were determined by RSM. The optimal medium was used to perform fermentation experiments by S. marcescens H30 in a 3.7 l bioreactor. Several feeding strategies including interim feeding, exponential feeding and constant residual sucrose concentration feeding were compared for improving 2,3-BD production. Ultimately, a suitable control strategy which combined the respiratory quotient (RQ) control with the constant residual sucrose concentration fed-batch was developed. Using this strategy, the maximum 2,3-BD concentration of 139.92 g/l with the diol (AC + BD) productivity of 3.49 g/l h and the yield of 94.67% was obtained. ? 2009 Elsevier Ltd.