文献类型：期刊文献

英文题名：Enhancing effect of Tween-80 on lipase performance in enantioselective hydrolysis of ketoprofen ester

作者：Liu, YY; Xu, JH; Hu, Y

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biocatalysis, Shanghai 200237, Peoples R China

年份：2000

卷号：10

期号：5

起止页码：523

外文期刊名：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

收录：;EI(收录号：2000105351212);WOS:【SCI-EXPANDED(收录号:WOS:000089363400008)】；

语种：英文

外文关键词：Candida rugosa lipase; enantioselectivity; activity; ketoprofen; Tween-80

摘要：An additive method has been developed to obtain nearly optically pure (S)-ketoprofen (S)-2-(3-benzoyphenyl)propionic acid, > 96% enantiomeric excess, eel by using lipase OF (from Candida rugosa) for the first time. The effect of surfactants (selected as additives) on the enantioselective hydrolysis of 2-chloroethyl ester of ketoprofen by the crude and the purified C. rugosa lipase (lipase OF) was investigated. Except for Tween-60, Tween-80 and nonyl phenol polyethyleneoxy ether (OP-10), most of the other surfactants tested had inhibitory influence on the lipase. As for the enantioselectivity, only Tween-80 displayed a positive effect. Moreover, the concentration of Tween-80 was found to be a sensitive factor affecting the activity and the enantioselectivity, when either the crude or the purified enzyme was used. Upon addition of the two emulsifiers at their optimal concentrations, i.e., 2% (w/v) Tween-80 and 3% (w/v) OP-10, the crude enzyme activity was greatly enhanced up to 13 and 15 times, respectively. On the other hand, the enantiomeric ratio (E value) increased from 1.2 to 6.7 for the crude lipase and from 8 to > 100 for the purified lipase in the presence of 8% or 2% (w/v) Tween-80. (C) 2000 Elsevier Science B.V. All rights reserved.