文献类型：期刊文献

中文题名：微生物酶法生产果糖低聚糖

英文题名：Production of Fructooligosaccharides by Microbial Enzyme System

作者：唐军[1];储炬[1];李友荣[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室

年份：1999

卷号：25

期号：4

起止页码：371

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2000045118548);Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：上海市自然科学基金

语种：中文

中文关键词：蔗果三糖;果糖低聚糖;微生物酶法;制备

外文关键词：fructooligosaccharides; 1kestose; enzymatic activity; enzymatic reaction

摘要：介绍了一种简便易行的制备蔗果三糖标准品的方法。研究得出曲霉６．２２２胞内、胞外酶均具有活性，最佳酶反应条件为：ｐＨ６．０，温度５０℃，时间６０ｍｉｎ。最佳初始蔗糖浓度为２５０ｇ／Ｌ。由曲霉６．２２２发酵制备的酶溶液稳定性良好。
It is available to prepare standard 1kestose easily. The intracellular and extracellular activities of the strain 6.222 (isolated from soil and identified as moulds) were investigated. The optimum enzymatic reaction conditions of it were as follows: pH6.0, 50, 60min. The optimal initial concentration of sucrose in enzymatic reaction is 250g/L. The stability of enzymatic solution is excellent.