文献类型：期刊文献

英文题名：A fluorescent probe for the discrimination between Cys and GSH

作者：Song, Lun[1];Sun, Qian[1];Wang, Nan[1];Chen, Zhaoyang[1];Zhang, Weibing[1];Qian, Junhong[1]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：7

期号：24

起止页码：10371

外文期刊名：ANALYTICAL METHODS

收录：;EI(收录号：20155001664712);WOS:【SCI-EXPANDED(收录号:WOS:000365912900038)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 21576085 and 21235005) and the Shanghai Municipal Natural Science Foundation (15ZR1409000).

语种：英文

外文关键词：Fluorescence spectroscopy - Amino acids - Lanthanum compounds - Probes

摘要：Fluorescent probe RTP was rationally designed and synthesized to differentially detect cysteine (Cys) and glutathione (GSH) in different channels. RTP was constructed by incorporating a nitrothiophenyl receptor to a rhodamine fluorophore. The replacement of the receptor by the mercapto group in biothiols forms a weakly fluorescent thioether. The amino group in Cys/Hcy further attacks the thioether through aromatic nucleophilic substitution via a five/six-membered ring to form highly fluorescent red-emitting amino-substituted rhodamine. Moreover, RTP has been successfully used for fluorescence imaging of Cys in living Hela cells.