文献类型：期刊文献

英文题名：Purification of flavonoids from licorice using an off-line preparative two-dimensional normal-phase liquid chromatography/reversed-phase liquid chromatography method

作者：Fan, Yunpeng[1];Fu, Yanhui[2];Fu, Qing[1];Cai, Jianfeng[1];Xin, Huaxia[1];Dai, Mei[1];Jin, Yu[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Hai Nan Normal Univ, Haikou, Peoples R China

年份：2016

卷号：39

期号：14

起止页码：2710

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20162802586707);WOS:【SCI-EXPANDED(收录号:WOS:000380728700006)】；

基金：This work was supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20130074120017) and the Natural Science Foundation of Shanghai, China (Grant No. 13ZR1453200).

语种：英文

外文关键词：Flavonoids; Licorice; Preparative high-performance liquid chromatography; Two-dimensional separation

摘要：An orthogonal (71.9%) off-line preparative two-dimensional normal-phase liquid chromatography/reversed-phase liquid chromatography method coupled with effective sample pretreatment was developed for separation and purification of flavonoids from licorice. Most of the nonflavonoids were firstly removed using a self-made Click TE-Cys (60 mu m) solid-phase extraction. In the first dimension, an industrial grade preparative chromatography was employed to purify the crude flavonoids. Click TE-Cys (10 mu m) was selected as the stationary phase that provided an excellent separation with high reproducibility. Ethyl acetate/ethanol was selected as the mobile phase owing to their excellent solubility for flavonoids. Flavonoids co-eluted in the first dimension were selected for further purification using reversed-phase liquid chromatography. Multiple compounds could be isolated from one normal-phase fraction and some compounds with bad resolution in one-dimensional liquid chromatography could be prepared in this two-dimensional system owing to the orthogonal separation. Moreover, this two-dimensional liquid chromatography method was beneficial for the preparation of relatively trace flavonoid compounds, which were enriched in the first dimension and further purified in the second dimension. Totally, 24 flavonoid compounds with high purity were obtained. The results demonstrated that the off-line two-dimensional liquid chromatography method was effective for the preparative separation and purification of flavonoids from licorice.