文献类型：期刊文献

英文题名：Interactions of biocidal guanidine hydrochloride polymer analogs with model membranes: a comparative biophysical study

作者：Zhou, Zhongxin[1];Zheng, Anna[2];Zhong, Jianjiang[1,3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Sch Bioengn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China

年份：2011

卷号：43

期号：9

起止页码：729

外文期刊名：ACTA BIOCHIMICA ET BIOPHYSICA SINICA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000294354700009)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (30821005 and 50573020) and the Shanghai Leading Academic Discipline Project (B203 and B505).

语种：英文

外文关键词：biocidal action mechanism; guanidine polymer; membrane interaction

摘要：Four synthesized biocidal guanidine hydrochloride polymers with different alkyl chain length, including polyhexamethylene guanidine hydrochloride and its three new analogs, were used to investigate their interactions with phospholipids vesicles mimicking bacterial membrane. Characterization was conducted by using fluorescence dye leakage, isothermal titration calorimetry, and differential scanning calorimetry. The results showed that the gradually lengthened alkyl chain of the polymer increased the biocidal activity, accompanied with the increased dye leakage rate and the increased binding constant and energy change value of polymer-membrane interaction. The polymer-membrane interaction induced the change of pretransition and main phase transition (decreased temperature and increased width) of phospholipids vesicles, suggesting the conformational change in the phospholipids headgroups and disordering in the hydrophobic regions of lipid membranes. The above information revealed that the membrane disruption actions of guanidine hydrochloride polymers are the results of the polymer's strong binding to the phospholipids membrane and the subsequent perturbations of the polar headgroups and hydrophobic core region of the phospholipids membrane. The alkyl chain structure significantly affects the binding constant and energy change value of the polymer-membrane interactions and the perturbation extent of the phospholipids membrane, which lead to the different biocidal activity of the polymer analogs. This work provides important information about the membrane disruption action mechanism of biocidal guanidine hydrochloride polymers.