文献类型：期刊文献

英文题名：Application of click chemistry on preparation of separation materials for liquid chromatography

作者：Chu, Changhu[1];Liu, Renhua[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2011

卷号：40

期号：5

起止页码：2177

外文期刊名：CHEMICAL SOCIETY REVIEWS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000289630700008)】；

基金：This work was supported by a grant from the National High Technology Research and Development Program of China (863 Program) (No. 2008AA06Z306). We are also grateful to Dr Xiaowen Sun for his kind discussion and help with language.

语种：英文

摘要：With the increasing requirement for analysis and separation of samples related to genomics, proteomics, metabolomics, pharmacology and agrochemistry, diverse stationary phases for liquid chromatography have been prepared by Cu(I)-catalyzed 1, 3-dipolar azide-alkyne cycloaddition reaction (CuAAC). It has been proved that CuAAC is a powerful tool for preparing covalently bonded stationary phases. In this tutorial review, we highlighted the preparation of separation materials by immobilization of functional groups on silica beads, polymer beads and agarose via CuAAC and their applications in liquid chromatography and related purposes, such as separation of polar compounds, enrichment of valuable bio-samples, orthogonal two-dimensional HPLC and chiral separation. Meanwhile, agarose-based separation materials for affinity chromatography are reviewed.