文献类型：期刊文献

中文题名：以CER为判据从摇瓶到反应器的ATP放大生产

英文题名：Study of ATP Production with Immobilized Saccharamyces Cerivisae Using Carbon Dioxide Evolution Rate as Scale up Criterion

作者：朱文洲[1];徐茜[1];邱蔚然[1];俞俊棠[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室

年份：1998

卷号：24

期号：1

起止页码：59

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;EI(收录号：1998054218594);Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：摇瓶;放大判据;固定化酵母;ATP;酶

外文关键词：shake flask; scale up criterion; immobilized saccharamyces cerivisae; ATP; carbon dioxide evolution rate( CER )

摘要：在Ｙ－Ｚ型多功能摇瓶中由固定化啤酒酵母将腺苷转化成ＡＴＰ，得到一组过程参数曲线；以摇瓶转化过程中的ＣＯ２释放率（ＣＥＲ）－转化时间曲线为参照曲线，在液流循环式固定化啤酒酵母流化床反应器中进行放大试验。对比实验结果，表明以ＣＥＲ为判据进行固定化啤酒酵母生产ＡＴＰ从摇瓶到反应器的放大是成功的。
The biotransformation of adenosine to ATP using immobilized saccharamyces cerivisae in multifunctional shake flask of Y Z type was studied and the process parameter curves were obtained. When the same process was scaled up into a liquid recycling fluidized bed reactor, it was found that of the time course profile of carbon dioxide evolution rates( CER ) was maintained identically with that obtained from sake flask test same conversion results could be obtained as that from shake flask test. So the CER can be considered as a sound scale up criterion of this biotransformation process.