文献类型：期刊文献

英文题名：Optimization of the n-propanol concentration and feedback control strategy with electronic nose in erythromycin fermentation processes

作者：Zhao, Hong-Tu;Pang, Kun-Yun;Lin, Wei-Lu;Wang, Ze-Jian[1];Gao, Da-Qi;Guo, Mei-Jin[1];Zhuang, Ying-Ping

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Inst Biomfg Technol, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Collaborat Innovat Ctr, Shanghai 200237, Peoples R China

年份：2016

卷号：51

期号：2

起止页码：195

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20155101708945);WOS:【SCI-EXPANDED(收录号:WOS:000370100200005)】；

基金：This work was financially supported by a Grant from the National Natural Science Foundation of China (Grant No. 31200024, 21176077 and 60675027), the Major State Basic Research Development Program of China (No. 2012AA021201 and 2015AA021005), the National Basic Research Program of China No. 2013CB733600.

语种：英文

外文关键词：Erythromycin; Fermentation; On-line n-propanol monitoring; Electronic nose; Feedback control

摘要：N-propanol is an important precursor in industrial antibiotic erythromycin fermentation by Saccharopolyspora erythraea. However, the online control of n-propanol concentration is a challenging problem, and hardly any effective methods have been applied in the fermentation process. In this study, an innovative method developed for on-line detection and feedback control of n-propanol concentration in erythromycin fermentation process with electronic nose. The models for n-propanol detection based on the response of the electronic nose were established, and the on-line monitoring feedback system for n-propanol concentration controlling strategy was applied in erythromycin fermentation. The effects of different residual n-propanol concentration in fermentation broth on physiological metabolism of S. erythraea were investigated in a 50-L bioreactor. The results revealed that maintaining an n-propanol concentration of 1000 +/- 38 ppm, detected by electronic nose, resulted in the highest erythromycin yield (8500 U/mL). In conclusion, real-time detection and feedback control of n-propanol concentration using electronic nose is an effective method for erythromycin fermentation processes and a new precursor adding optimization process was developed. (C) 2015 Published by Elsevier Ltd.