文献类型：期刊文献

英文题名：Correspondence on "Structural Insight into the Catalytic Mechanism of the Endoperoxide Synthase FtmOx1"

作者：Zhang, Lixin[1];Liu, Xueting[1];Wang, Xinye[1];Zhu, Guoliang[1];Song, Heng[2];Cheng, Ronghai[3];Naowarojna, Nathchar[3];Costello, Catherine E. E.[3];Liu, Pinghua[3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China;[3]Boston Univ, Dept Chem, Boston, MA 02215 USA

年份：2023

卷号：62

期号：37

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20233214492023);WOS:【SCI-EXPANDED(收录号:WOS:001041731000001)】；

基金：Acknowledgments We thank Prof. Wupeng Yan and Dr. Andrew Weitz for critical comments on this manuscript. This work was supported in part by grants from the National Natural Science Foundation (31720103901 to L.Z.) and the National Institute of Health (GM140040 to P.L.).

语种：英文

外文关键词：Biosynthesis; Endoperoxides; FtmOx1; Iron; Non-heme enzymes

摘要：In their recent Angewandte Chemie publication (doi: 10.1002/anie.202112063), Cen, Wang, Zhou et al. reported the crystal structure of a ternary complex of the non-heme iron endoperoxidase FtmOx1 (PDB entry 7ETK). The biochemical data assessed in this study were from a retracted study (doi: 10.1038/nature15519) by Zhang, Liu, Zhang et al.; no additional biochemical data were included, yet there was no discussion on the source of the biochemical data in the report by Cen, Wang, Zhou et al. Based on this new crystal structure and subsequent QM/MM-MD calculations, Cen, Wang, Zhou et al. concluded that their work provided evidence supporting the CarC-like mechanistic model for FtmOx1 catalysis. However, the authors did not accurately describe either the CarC-like model or the COX-like model, and they did not address the differences between them. Further, and contrary to their interpretations in the manuscript, the authors' data are consistent with the COX-like model once the details of the CarC-like and COX-like models have been carefully analyzed.