文献类型：期刊文献

英文题名：Cauliflower-Like Nanostructured Localized Surface Plasmon Resonance Biosensor Chip for Cytokine Detection

作者：Luo, Xi[1,2];Zhu, Chen[2];Saito, Masato[1,2];Espulgar, Wilfred Villariza[2];Dou, Xiaoming[2,3];Terada, Yuhei[1,2];Obara, Ain[1,2];Uchiyama, Sachiyo[1];Tamiya, Eiichi[1,2]

机构：[1]Osaka Univ, AIST, Adv Photon & Biosensing Open Innovat Lab, Suita, Osaka 5650871, Japan;[2]Osaka Univ, Grad Sch Engn, Dept Appl Phys, Suita, Osaka 5650871, Japan;[3]East China Univ Sci & Technol ECUST, Grad Sch Sci, Inst Photon & Biomed IPBM, Shanghai 200237, Peoples R China

年份：2020

卷号：93

期号：9

起止页码：1121

外文期刊名：BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN

收录：;EI(收录号：20210509858869);WOS:【SCI-EXPANDED(收录号:WOS:000566446900005)】；

基金：This work was supported by Grant-in-Aid for Scientific Research (Kiban S, No. 15H05769) and JST CREST Grant (No. JPMJCR16G2), Japan. A part of this work was supported by "Nanotechnology Platform Project (Nanotechnology Open Facilities in Osaka University)" of Ministry of Education, Culture, Sports, Science and Technology, Japan (No. S-19-OS0045). Thank to Dr. Shohei Koyama, Dr. Atsushi Kumanog, and Dr. Hyota Takamatsu from Immunology Frontier Research Center of Osaka University for providing IL-6 over-expressed Jurkat cells.

语种：英文

外文关键词：Cauliflower-like nanostructure; Localized surface plasmon resonance; Cytokine detection

摘要：Localized surface plasmon resonance (LSPR) based nanoplasmonic biosensors have attracted great attentions due to rapid detection and label-free capability. Aiming to obtain a high performance LSPR sensor chip, a cauliflower-like nanopillar (CLNP) structure was fabricated based on the cyclo-olefin polymer (COP) material which could increase the sensitivity according to the "hotspots" effect theory. An oxygen plasma etching procedure was introduced to the chip fabrication process to carve the nanopillar surface prepared by nanoimprinting lithography (NIL) into the cauliflower-like nanostructures. In this paper, the cauliflower-like nanopillar structured sensor chip was successfully obtained and the results confirmed that this sensor chip has higher sensitivity compared to an untreated nanopillar structured chip. Meanwhile, the biosensing capability was verified by cell interleukin-6 (IL-6) immunoassay. This approach provides an easy way to fabricate mass-producible LSPR biosensor chips for cell cytokine secretion detection.