文献类型：期刊文献

英文题名：Purification of Ε-polylysine from fermentation broth by a new method of metal ion complexation

作者：Sui, Yucui[1]; Yang, Ting[2]; Zhao, Yangyang[1]; Wan, JunFen[1]; Cao, Xuejun[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, Shanghai, 200237, China; [2] State Key Laboratory of Metal Matrix Composites, School of Material Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China

年份：2023

卷号：130

起止页码：512

外文期刊名：Process Biochemistry

收录：EI(收录号：20232114116073)

语种：英文

外文关键词：Activated carbon - Antimicrobial agents - Fermentation - Infrared spectroscopy - Metal ions - Microorganisms - Molecular modeling - Precipitation (chemical)

摘要：Ε-Polylysine (Ε-PL), an efficient and safe natural biological preservative, has shown a promising development. However, the downstream purification of Ε-PL remains a bottleneck due to high costs and low yields. This study aimed to develop a new approach of metal ion complexation for the purification of Ε-PL from fermentation broth. The purification procedure was optimized and the effect of Ni2+ and Zn2+ on the purification of Ε-PL was investigated. The results indicated that the optimal conditions for Ε-PL purification included complexation with 130 mM metal ion at pH 9.5, acid dissolution at pH 2, decolorization with 1 % (w/v) activated carbon, and precipitation with 80 % ethanol. Compared with Zn2+, Ni2+ as complexing agent had a better purification effect on Ε-PL. The final purity and recovery of Ε-PL sample obtained by Ni2+ could reach 98.16 % and 81.27 %, while also meeting the requirement of antimicrobial. Furthermore, infrared spectroscopy and molecular docking were used to analyze the coordination regions of Ε-PL and metal ions. The results showed that the coordination regions of Ε-PL were amino, carbonyl and carboxyl groups, and molecular docking provided a theoretical basis for the reasonable application of different metal ions in the purification process of Ε-PL. ? 2023 Elsevier Ltd