文献类型：期刊文献

英文题名：Purification, structural characterization, and anti-ulcerative colitis activity of a novel pectin-like polysaccharide AH-H4-1 from Alhagi honey

作者：Wang, Zhe[1];Chi, Yaping[1];Wang, Menghua[3];Zhou, Qianqian[1];Yan, Zexiang[1];Xue, Rui[1];Cai, Xiaoxia[1];Tian, Jing[1];Yu, Wei[1];Yaermaimaiti, Saimijiang[1];Turghun, Chimengul[1];Sun, Yakun[1];Song, Wangdi[1];Du, Daohai[4];Luo, Cheng[4];Hu, Zelan[2];Han, Bo[1]

机构：[1]Shihezi Univ, Inst Safflower Ind Res, Sch Pharm,Minist Educ, Key Lab Xinjiang Phytomed Resource & Utilizat, Shihezi 832002, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[3]Shandong Univ, Sch Pharmaceut Sci, Jinan 250012, Peoples R China;[4]Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China

年份：2025

卷号：319

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20252718706841);WOS:【SCI-EXPANDED(收录号:WOS:001533249800001)】；

基金：This work was supported by the Grant from the Financial science and technology plan project of Xinjiang (2023AB047, 2022A03017) , Na-tional Natural Science Foundation of China (82174075) .

语种：英文

外文关键词：Alhagi honey; Pectin-like polysaccharide; DSS-induced UC model; Structural characterization

摘要：Alhagi honey (AH) has been employed in traditional medicine for treating intestinal diseases, demonstrating significant clinical efficacy. However, it remains unclear which components of AH play a role in the treatment of ulcerative colitis (UC). Here, high molecular weight polysaccharide (AH-H) and low molecular weight oligosaccharide (AH-L) were obtained from AH by dialysis. AH-H showed significant therapeutic activity in dextran sulfate sodium (DSS)-induced UC mouse model. In vitro experiment indicated that the component AH-H4-1 isolated from AH-H was a potential active ingredient. Structure analysis revealed that AH-H4-1 was primarily composed of galacturonic acid, rhamnose, galactose, and arabinose. Its structural backbone consists of 1,4-GalA, 1,2,4-Rha, and 1,2-Rha residues, with branches at the O-4 position of 1,2,4-Rha containing 1,3,6-Gal, 1,6-Gal, and 1,5-Ara. AH-H4-1 demonstrates therapeutic effects by reducing neutrophil accumulation in the intestine and promoting the repair of intestinal barrier function in zebrafish with DSS-induced UC. This study provides a valuable reference for AH in the treatment of UC, and establishes a phytochemical basis for developing AHderived therapeutics for the treatment of UC.