文献类型：期刊文献

英文题名：Probing the degradation mechanism of forsythiaside A and simultaneous determination of three forsythiasides in Forsythia preparations by a single marker

作者：Qi, Minghui[1];Zhao, Shiyi[1];Zhou, Bin[1];Zhang, Min[2,3];Zhang, Hongyang[1];Wang, Yuerong[1];Hu, Ping[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai, Peoples R China

年份：2019

卷号：42

期号：23

起止页码：3503

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20194507622877);WOS:【SCI-EXPANDED(收录号:WOS:000492272300001)】；

基金：This research was financially supported by the National Natural Science Foundation of China (No. 81973285 and No. 81573397).

语种：英文

外文关键词：degradation pathway; forsythiasides; mass spectrometry; traditional Chinese medicine; ultra high performance liquid chromatography

摘要：Forsythiaside A is the major component of Forsythia suspensa. This study investigated the degradation mechanism of forsythiaside A. Eight degraded components including forsythiaside I, forsythiaside H, forsythiaside E, caffeic acid, suspensaside A, beta-hydroxy forsythiaside I, beta-hydroxy forsythiaside H, and beta-hydroxy forsythiaside A were identified by using ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry. Then, the quantitative analysis of multi-components by a single-marker was performed with ultra-high performance liquid chromatography to simultaneously determine forsythiaside A, forsythiaside H, and forsythiaside I in Forsythia suspensa preparations. The result showed good linear relationships within 2.871-287.1, 0.231-23.1, and 0.983-98.3 mu g/mL (r > 0.9998), with average recoveries of 97.7, 95.7, and 95.8% and relative standard deviations of 1.4, 2.4, and 1.8%, respectively. Using forsythiaside A as an internal reference, the relative retention values of forsythiaside H and forsythiaside I to forsythiaside A were calculated to be 0.89 and 0.61, respectively, and the relative correction factors were 0.816 and 0.799, respectively. The method for quantitative analysis of multi-components by a single-marker was applied to evaluate the overall quality of forsythia preparations. There was no significant difference in the measurement results of the method developed and the method of external standard.