文献类型：期刊文献

英文题名：Long-circulating polymeric nanoparticles bearing a combinatorial coating of PEG and water-soluble chitosan

作者：Sheng, Yan[1];Liu, Changsheng[1,2];Yuan, Yuan[3];Tao, Xinyi[1];Yang, Fan[2];Shan, Xiaoqian[3];Zhou, Huanjun[1];Xu, Feng[3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, PR, Peoples R China;[2]E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, PR, Peoples R China;[3]E China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, PR, Peoples R China

年份：2009

卷号：30

期号：12

起止页码：2340

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20090911928548);WOS:【SCI-EXPANDED(收录号:WOS:000264512800019)】；

基金：The authors acknowledge the financial support from the National High Technology Research and Development Program of China (863 program) (No. 2004AA-302050) and from Shanghai Nanotechnology Special Foundation (No. 0452nm022).

语种：英文

外文关键词：Polymeric nanoparticles; Polyethylene glycol; Water-soluble chitosan; Long-circulating; Biodistribution

摘要：A major obstacle in the development of polymeric nanoparticles (NPs) as effective drug delivery vesicles is the rapid clearance from blood. In order to realize a significant prolongation in blood circulation, a combinatorial design, covalent attachment of polyethylene glycol (PEG) to polylactic acid (PLA) and physical adsorption of water-soluble chitosan (WSC) to particle surface, has been developed for surface modification of PLA NPs. Two types of WSC, cationic partially deacetylated chitin (PDC) and anionic N-carboxy propionyl chitosan sodium (CPCTS) were investigated. All the NPs formulated in the size range of 100-200 nm were prepared by a modified w/o/w technique and physicochemically characterized. In vitro phagocytosis by mouse peritoneal macrophage (MPM), in vivo blood clearance and biodistribution following intravenous administration in mice, of these NPs labeled with 6-coumarin, were evaluated. The presence of WSC. whether alone or with PEG, highly improved the surface hydrophilicity as well as suspension stability of NPs. Their surface charge was greatly affected by the WSC coating, being close to neutrality for PEG/PDC NPs and highly negative in the case of PEG/CPCTS NPs. In comparison to NPs treated with PEG or WSC alone, the synergistic action of PEG and WSC strongly inhibited the macrophage uptake and extended the circulation half-life (t(1/2)) with concomitant reduced liver sequestration. Particularly, PEG/PDC NPs showed the most striking result with regard to their performance in vitro and in vivo. Calculated t(1/2) of PEG/PDC NPs and PEG/CPCTS NPs was 63.5 h and 7.1 h, respectively, much longer than that of control PEG/PVA NPs (1.1 h). More WSC materials need to be evaluated, but the present data suggest that, a combinatorial coating of PEG and PDC greatly prolongs the systemic circulation of NPs and represents a significant step in the development of long-circulating drug delivery carriers. (C) 2008 Elsevier Ltd. All rights reserved.