文献类型：期刊文献

英文题名：Versatile Probes for the Selective Detection of Vicinal-Dithiol-Containing Proteins: Design, Synthesis, and Application in Living Cells

作者：Huang, Chusen[1,2];Yin, Qin[2];Meng, Jiangjiang[2];Zhu, Weiping[1];Yang, Yi[2];Qian, Xuhong[1,2];Xu, Yufang[1,2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：19

期号：24

起止页码：7739

外文期刊名：CHEMISTRY-A EUROPEAN JOURNAL

收录：;EI(收录号：20132416408677);WOS:【SCI-EXPANDED(收录号:WOS:000319825500014)】；

基金：We thank the financial support from National Natural Science Foundation of China (Grants 21076077, 21236002), the National Basic Research Program of China (973 Program, 2013CB733700, 2010CB126100), the National High Technology Research and Development Program of China (863 Program, 2011AA10A207), Shanghai Pujiang Program and the Fundamental Research Funds for the Central Universities, The Innovative Program of Shanghai Normal University (SK201331), the Science and Technology Innovation Foundation for College Students (B-7062-12-001114).

语种：英文

外文关键词：conjugation; fluorescent probes; imaging agents; redox; vicinal dithiols

摘要：Endogenous vicinal-dithiol-containing proteins (VDPs) that have two thiol groups close to each other in space play a significant importance in maintaining the cellular redox microenvironment. Approaches to identify VDPs mainly rely on monitoring the different concentration of monothiol and total thiol groups or on indirect labeling of vicinal thiols by using p-aminophenylarsenoxide (PAO). Our previous work has reported the direct labeling of VDPs with a highly selective receptor PAO analogue, which could realize fluorescence detection of VDPs directly in living cells. Herein, we developed a conjugated approach to expand detectable tags to nitrobenzoxadiazole (NBD), fluorescein, naphthalimide, and biotin for the synthesis of a series of probes. Different linkers have also been introduced toward conjugation of VTA2 with these functional tags. These synthesized flexible probes with various features will offer new tools for the potential identification and visualization of vicinal dithiols existing in different regions of VDPs in living cells. These probes are convenient tools for proteomics studies of various disease-related VDPs and for the discovery of new drug targets.