文献类型：期刊文献

英文题名：Improved vitamin B12 production by step-wise reduction of oxygen uptake rate under dissolved oxygen limiting level during fermentation process

作者：Wang, Ze-Jian[1]; Wang, Hui-Yuan[1]; Li, Yong-Liang[2]; Chu, Ju[1]; Huang, Ming-Zhi[1]; Zhuang, Ying-Ping[1]; Zhang, Si-Liang[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China; [2] Shijiazhuang Pharma, Group Hua Rong Pharmaceutical Co. Ltd., Shijiazhuang, 050041, China

年份：2010

卷号：101

期号：8

起止页码：2845

外文期刊名：Bioresource Technology

收录：EI(收录号：20100212627958)

语种：英文

外文关键词：Fermentation - Bacteria - Biosynthesis - Cell growth - Process control - Oxygen supply - Biochemistry - Growth kinetics

摘要：Effects of different oxygen transfer rates (OTR) on the cell growth and vitamin B12 biosynthesis of Pseudomonas denitrificans were first investigated under dissolved oxygen limiting conditions. The results demonstrated that high OTR accelerated cell growth and initial vitamin B12 biosynthesis rate, while lower OTR was critical for higher productivity in the late fermentation process. The oxygen uptake rates (OUR) corresponded well with OTR. Based on the metabolic intermediate analysis, a step-wise OUR control strategy was proposed. The strategy was successfully implemented in scale-up to an industrial fermenter (120,000 l). A stable maximum vitamin B12 production of 208 ± 2.5 mg/l was achieved, which was increased by 17.3% compared with the control. Furthermore, the glucose consumption coefficient to vitamin B12 was 34.4% lower than that of the control. An efficient and economical fermentation process based on OUR criterion was established for industrial vitamin B12 fermentation by P. denitrificans. ? 2009.