文献类型：期刊文献

英文题名：Cloning and expression of a novel lipase gene from Pseudomonas fluorescens B52

作者：Jiang, Zhengbing[1]; Zheng, Yitao[1]; Luo, Yu[1]; Wang, Gang[1]; Wang, Hongping[1]; Ma, Yushu[1]; Wei, Dongzhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China

年份：2005

卷号：31

期号：2

起止页码：95

外文期刊名：Molecular Biotechnology

收录：EI(收录号：2005389378717)

语种：英文

外文关键词：DNA - Fluorescence - Fungi - Genes

摘要：A novel lipase gene (lipB52) was isolated directly from the genomic DNA of Pseudomonas fluorescens B52 with the genome-walking method, an effective method for isolating lipase gene from bacteria. There was an open reading frame (ORF) of 1854 bp, which encoded 617 amino acids. The lipase gene (lipB52) was cloned into expression vector pPIC9K and successfully integrated into a heterologous fungal host, Pichia pastoris KM71, and the recombinant Pichia pastoris were screened with a high throughput method. The recombinant was induced by methanol to secrete active lipase into the culture medium. The recombinant lipase LipB52 was also purified and characterized. The optimum temperature for the purified lipase LipB52 was 40°C at pH 8.0. It exhibited better thermos lability and pH stability than its homologs. ? 2005 Humana Press Inc. All rights of any nature whatsoever reserved.