文献类型：期刊文献

中文题名：农用抗真菌海洋微生物的筛选及放线菌T19-07活性代谢产物的初步研究

英文题名：Screening of marine microorganisms with agricultural antifungal activities and preliminary study on the bioactive metabolites produced by strain T19-07

作者：胡杨[1];张道敬[1];李元广[1];陶黎明[2];田黎[3];李淑兰[4]

机构：[1]生物反应器工程国家重点实验室华东理工大学,上海200237;[2]上海市化学生物学重点实验室华东理工大学药学院,上海200237;[3]国家海洋局第一海洋研究所,山东青岛266003;[4]上海泽元海洋生物技术有限公司,上海200237

年份：2011

卷号：30

期号：4

起止页码：12

中文期刊名：中国海洋药物

外文期刊名：Chinese Journal of Marine Drugs

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

基金：国家863高科技发展计划(No.2008AA09Z407)

语种：中文

中文关键词：海洋放线菌;植物病原真菌;抗真菌活性

外文关键词：marine actinomyces; phytopathogenic fungi; antifungal activity

摘要：目的通过筛选获得具有拮抗植物病原真菌活性的海洋微生物菌株,并对其中一株海洋生境的结节链霉菌(Streptomyces nodosus)T19-07的活性代谢产物进行初步研究。方法以植物病原真菌为靶标,采用平板对峙培养法筛选出活性菌株;再以抑菌活性为指标,考察较强活性菌株T19-07在5L发酵罐中的培养过程特征,并通过大孔吸附树脂XAD-16柱层析对活性物质进行分离提取,结合TLC生物自显影和HPLC快速确定代谢产物中的活性组分。结果从31株海洋微生物中筛选出12株对多种植物病原真菌具有拮抗作用的菌株;确定了菌株T19-07的代谢产物中的主要抑菌活性物质,其相对分子质量为214,并且它对茄交链格孢霉的离体抑菌活性和已登记的化学农药异菌脲活性相当,MIC<12.5μg.mL-1。结论海洋微生物是寻找农用抗生素的重要资源,海洋放线菌T19-07产生的拮抗植物病原真菌物质具有较好的离体抑菌活性,具有进一步研究开发的潜力。
Objective To screen the active strains with agricultural antifungal activity from marine microorganisms as well as the bioactive metabolites produced by marine actinomyces Streptomyces nodosus T19-07.Methods Using phytopathogenic fungi as targets,the active strains were screened out by pairing culture assay.Based on the index of antifungal activity,the characteristics of fermentation of strain T19-07 in 5 L fermentor were studied,and the bioactive substances were extracted by macroporous resin XAD-16,and TLC bioautography combined with HPLC was used to separate the active compound.Results Twelve antagonistic strains against phytopathogenic fungi were screened out from 31 marine microorganisms.The main antifungal substance of strain T19-07 was isolated with relative molecular mass of 214 Da,and its antifungal activity in vitro against Alternaria solani was similar with iprodione registrated as a chemical pesticide,MIC below 12.5 μg·mL-1.Conclusion Marine microorganisms are important resources to find agricultural antibiotics.The bioactive substances produced by marine actinomyces S.nodosus T19-07 exhibit potent antifungal activity in vitro,and it has the potential for further study.