文献类型：期刊文献

英文题名：Oxidation of ethylene glycol to glycolaldehyde using a highly selective alcohol dehydrogenase from Gluconobacter oxydans

作者：Zhang, Xingxing[1];Zhang, Baoqi[1];Lin, Jinping[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：112

起止页码：69

外文期刊名：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

收录：;EI(收录号：20160501877702);WOS:【SCI-EXPANDED(收录号:WOS:000349592700010)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 21276084/B060804), "973" Program (No. 2012CB721003), National major science and technology projects of China (No. 2012ZX09304009).

语种：英文

外文关键词：Alcohol dehydrogenase; NADH oxidase-2; Ethylene glycol; Glycolaldehyde; Cofactor regeneration

摘要：A cytosolic medium-chain alcohol dehydrogenase Gox0313 from Gluconobacter oxydans DSM2003 was heterologously expressed in Escherichia coli BL21 (DE3) and the resulting proteins were purified and characterized. The recombinant enzyme was confirmed to have a good ability to selectively oxidize the terminal hydroxyl group of varied aliphatic and aromatic diols to the corresponding hydroxyl aldehydes, with no oxidative activities toward hydroxyl aldehydes in the presence of NAD(+). This enzyme could not oxidize sec-alcohols. Among the primary diols, ethylene glycol was efficiently converted to glycolaldehyde by Gox0313 coupled with NADH oxidase-2 (NOX-2) from Lactobacillus brevis ATCC367 for NAD(+) regeneration. Under the optimal conditions using this enzyme-coupled system (1 U/mL Gox0313 and 4 U/mL NOX-2), 42.32 mM glycolaldehyde was produced from 50 mM ethylene glycol in 8 h with a yield of 83.2%. When the enzyme concentration of Gox0313 was increased to 120 U/mL in the reaction, 500 mM of ethylene glycol was converted to 484.2 mM of glycolaldehyde in 14 h, resulting in a yield of 96.8%. With respect to the superior catalytic activity to primary diols, Gox0313 might be a potentially promising biocatalyst for bioproduction of glycolaldehyde and other hydroxyl aldehydes. (C) 2014 Elsevier B.V. All rights reserved.