文献类型：期刊文献

中文题名：高分子量透明质酸的发酵研究

英文题名：Fermentation Conditions of Hyaluronic Acid with High Molecular Weight

作者：田毅红[1];蔡海波[1];陆仕灿[1];谭文松[1];许建和[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2004

卷号：35

期号：5

起止页码：269

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：透明质酸;分批补料发酵;兽疫链球菌

外文关键词：hyaluronic acid; fed-batch fermentation; Streptococcus zooepidemicus

摘要：采用兽疫链球菌(Streptococcus zooepidemicus)进行分批补料发酵生产透明质酸(HA)。结果表明:采用葡萄糖初始浓度为4%,分批补加葡萄糖,使其浓度控制在2%,可使HA的产量达到3.4g/L,平均分子量2.45×106Da。若加入0.75g/L尿苷一磷酸,HA的产量可提高20.6%,达4.1g/L,平均分子量2.87×106Da。
Optimization of the fed-batch fermentation process of hyaluronic acid (HA)produced by Streptococcuszooepidemicus was studied. The initial concentration of glucose was 4% and then was controlled at 2% by feeding. Theyield of HA was raised to 3.4g/L and the average molecular weight was 2.45×106Da. When the initial medium supplementedwith exogenous uridine monophosphate, the yield of HA reached 4.1g/L and the average molecular weight was 2.87×106Da.