文献类型：期刊文献

英文题名：The cross-regulation between two-component system BasS-BasR and ferric uptake regulator Fur in virulence gene expression in Edwardsiella piscicida

作者：Ahmed, Moamer A. H.[1];Cai, Jingxiao[1];Zhang, Yuanxing[3,4];Yin, Kaiyu[2];Wang, Qiyao[1,4];Shao, Shuai[1,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Business Sch, Sch Hospitality Management, Shanghai 200235, Peoples R China;[3]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[4]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2022

卷号：559

外文期刊名：AQUACULTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000811613200008)】；

基金：This work was supported by grants from National Natural Science Foundation of China (32002436 to SS, 32102852 to YKY) , National Key Research and Development Program (2022YFE0101200) , China Agri-culture Research System of MOF and MARA (CARS-47) , and the Fundamental Research Funds for the Central Universities (22221062017019) .

语种：英文

外文关键词：Edwardsiella piscicida; EsrB; BasS-BasR TCS; Iron uptake; Virulence

摘要：Edwardsiella piscicida is a widespread pathogen that infects a vast variety of fish species and causes hemorrhagic septicemia and significant property damage in the global aquaculture industry. E. piscicida employs type III and VI secretion systems (T3/T6SS) to penetrate and colonize macrophages, which are regulated by the EsrA-EsrB two-component system (TCS). In this study, we demonstrate that the BasS-BasR TCS positively regulates the expression of esrB and controls the secretion of T3/T6SS proteins. The regulatory protein BasR bound to the esrB promoter to activate the expression of virulence genes. Upon sensing the environmental ferric signals, BasS-BasR activated virulence gene expression through EsrB and simultaneously released Fur repression on esrB expression, eventually promoting E. piscicida colonization in turbot. Overall, this study revealed a new role of BasS-BasR TCS in virulence regulation and iron uptake, providing valuable insights into bacterial parthenogenesis.