文献类型：期刊文献

英文题名：Single-pot conversion of cephalosporin C to 7-aminocephalosporanic acid in the absence of hydrogen peroxide

作者：Tan, Qiang[2,3];Zhang, Yewang[2,4];Song, Qingxun[2];Wei, Dongzhi[1,2]

机构：[1]E China Univ Sci & Technol, Inst Biochem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Guangxi Univ Tradit Chinese Med, Fac Pharm, Nanning 530001, Peoples R China;[4]Jiangsu Univ, Fac Pharmaceut Engn Dept, Zhenjiang 212013, Peoples R China

年份：2010

卷号：26

期号：1

起止页码：145

外文期刊名：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

收录：;EI(收录号：20101712878969);WOS:【SCI-EXPANDED(收录号:WOS:000273481800018)】；

基金：We thank Professor Zhongyi Yuan for kindly providing the recombinant P. pastoris strains. We are also grateful to Dr Tianwen Wang for reading the manuscript. This study was supported by the Key Disciplinary Foundation of Shanghai.

语种：英文

外文关键词：Cephalosporin C; D-Amino acid oxidase; Glutaryl-7-aminocephalosporanic acid acylase; Catalase; 7-Aminocephalosporanic acid

摘要：In this study, D-amino acid oxidase (DAAO) and catalase (CAT) in the permeabilized recombinant Pichia pastori cells were well investigated. It appeared that their thermal stability was negatively correlated with the apparent enzymatic activities. The frozen-melted cells presented the best stability and the lowest apparent activities of DAAO and CAT, whereas the cetyltrimethylammonium bromide (CTAB) permeabilized cells displayed the weakest stability and the highest apparent activities of the two enzymes. Simultaneous action of DAAO and CAT in the CTAB-permeabilized cells and glutaryl-7-aminocephalosporanic acid acylase (GA) immobilized on carrier contributed to the conversion of cephalosporin C (CPC) to 7-aminocephalosporanic acid (7-ACA) with a yield of 76.2%. During such a reaction cycle, no visible activity loss occurred at the immobilized GA, whereas the loss rates of DAAO and CAT activities were about 0.029 and 1.13 U min(-1), respectively. Nevertheless, this problem could be easily solved by continuous feeding of the new permeabilized cell suspension at the rate of 6 ml h(-1) to the reactor. Following such a fed-batch strategy, these permeabilized cells and the immobilized GA could be efficiently reused for 6 and 15 reaction cycles, respectively, yielding around 76% 7-ACA at each reaction cycle.