文献类型：期刊文献

英文题名：Antibacterial effects of cinnamon (Cinnamomum zeylanicum) bark essential oil on Porphyromonas gingivalis

作者：Wang, Yue[1];Zhang, Yi[2];Shi, Yan-qin[1];Pan, Xian-hua[1];Lu, Yan-hua[2];Cao, Ping[3]

机构：[1]Shanghai Inst Technol, Shanghai 201418, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Shanghai Jahwa Grp Co Ltd, Shanghai 200082, Peoples R China

年份：2018

卷号：116

起止页码：26

外文期刊名：MICROBIAL PATHOGENESIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000429085200005)】；

基金：The authors received financial support from Shanghai Jahwa (Group) Co., Ltd. (Shanghai, China) (No. 169008) as collaborative research.

语种：英文

外文关键词：Cinnamon bark essential oil; Cinnamaldehyde; Porphyromonas gingivalis; Periodontal disease; Antibacterial mechanism

摘要：The objective of this study was to investigate the antibacterial effects of cinnamon (Cinnamomum zeylanicum) bark essential oil (CBEO) and its principal constituent cinnamaldehyde against Porphyromonas gingivalis and to elucidate the antibacterial mechanism. GC-MS analysis showed that cinnamaldehyde was the major constituent in CBEO (57.97%). The minimum inhibition concentrations (MICs) of CBEO and cinnamaldehyde were 6.25 mu g/mL and 2.5 mu M for P. gingivalis, respectively. Nucleic acid and protein leakage was observed with increasing concentrations of CBEO and cinnamaldehyde. Additionally, propidium iodide uptake assays revealed CBEO and cinnamaldehyde at 1 x MIC impaired P. gingivalis membrane integrity by enhancing cell permeability. Morphological changes in P. gingivalis cells were observed by scanning electron microscopy, which indicated cell membrane destruction. To further determine the anti-biofilm effect, relative biofilm formation and established biofilms were examined, which demonstrated that both CBEO and cinnamaldehyde at sub-MIC levels inhibited P. gingivalis biofilm formation by 74.5% and 67.3% separately, but only CBEO slightly decreased established biofilms by 33.5% at 4 x MIC. These results suggest the potential of CBEO as a natural antimicrobial agent against periodontal disease. Furthermore, cinnamaldehyde was confirmed to be the antibacterial substance of CBEO with inhibitory action against P. gingivalis.