文献类型：期刊文献

英文题名：Simultaneous Saccharification of Inulin and Starch Using Commercial Glucoamylase and the Subsequent Bioconversion to High Titer Sorbitol and Gluconic Acid

作者：An, Kehong[1];Hu, Fengxian[1];Bao, Jie[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：171

期号：8

起止页码：2093

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;EI(收录号：20140617273232);WOS:【SCI-EXPANDED(收录号:WOS:000327494800015)】；

基金：This research was supported by the National Basic Research Program of China (2011CB707406), the National High-Tech Program of China (2012AA022301), the Fundamental Research Funds for the Central Universities of China (WF0913005/1114054), and the Shanghai Leading Academic Discipline Project (B505).

语种：英文

外文关键词：Inulin; Starch; Glucoamylase; Sorbitol; Immobilized Zymomonas mobilis

摘要：A new bioprocess for production of sorbitol and gluconic acid from two low-cost feedstocks, inulin and cassava starch, using a commercially available enzyme was proposed in this study. The commercial glucoamylase GA-L NEW from Genencor was found to demonstrate a high inulinase activity for hydrolysis of inulin into fructose and glucose. The glucoamylase was used to replace the expensive and not commercially available inulinase enzyme for simultaneous saccharification of inulin and starch into high titer glucose and fructose hydrolysate. The glucose and fructose in the hydrolysate were converted into sorbitol and gluconic acid using immobilized whole cells of the recombinant Zymomonas mobilis strain. The high gluconic acid concentration of 193 g/L and sorbitol concentration of 180 g/L with the overall yield of 97.3 % were obtained in the batch operations. The present study provided a practical production method of sorbitol and gluconic acid from low cost feedstocks and enzymes.