文献类型：期刊文献

中文题名：亲和层析纯化云芝糖肽

英文题名：Purification of Polysaccharide Peptide of Coriolus Versicolor in Affinity Chromatography

作者：雷引林[1];王晓东[1];刘坐镇[1];邬行彦[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2001

卷号：23

期号：1

起止页码：8

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：CSTPCD;;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金项目!编号 2 970 6 0 0 2

语种：中文

中文关键词：云芝糖肽;亲和层析;分离纯化

外文关键词：Polysaccharide peptide of Coriolus versicolor ; Affinity Chromatography; Separation and purification<

摘要：目的 :探讨硼配基亲和层析纯化云芝糖肽的新方法。方法 :采用静态方法研究吸附过程的热力学、动力学、最佳吸附解吸条件 ,依据Chase模型求出最大静态吸附容量qm 和吸附常数Kd。结果 :qm=5 5 .5 7mg/g湿基 ,Kd=5 .312g/L ,动态吸附容量 :43 .1mg多糖 /g湿基 ,10 .3mg蛋白 /g湿基。 结论
Objective: To study a new purification method of Polysaccharide Peptide of Coriolus versicolor in affinity chromatography. Methods: Isotherm and kinetics in absorption and the optimal conditions of absorption and elution were studied through static experiments. The static absorption capacity q m and absorption constant K d were calculated according to Chase model. Results: q m =55.57mg/g wet resin, K d =5.312g/L, the dynamic absorption capacity is 43.1mg, polysaccharide/g wet resin and 10.3mg protein/g wet resin.Conclusion: Affinity chromatography can be used to purify PSP preliminarily.