文献类型：期刊文献

英文题名：Simultaneous saccharification and high titer lactic acid fermentation of corn stover using a newly isolated lactic acid bacterium Pediococcus acidilactici DQ2

作者：Zhao, Kai[1];Qiao, Qingan[1];Chu, Deqiang[1];Gu, Hanqi[1];Dao, Thai Ha[1];Zhang, Jian[1];Bao, Jie[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：135

起止页码：481

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20131716246336);WOS:【SCI-EXPANDED(收录号:WOS:000319181000068)】；

基金：This research was supported by National Basic Research Program of China (2011CB707406), National High-Tech Program of China (2012AA022301), Natural Science Foundation of China (20976051), China Postdoctoral Science Foundation (2011M500742/2012T50380), the Fundamental Research Funds for the Central Universities of China (WF0913005/WF1114054), and the Shanghai Leading Academic Discipline Project (B505).

语种：英文

外文关键词：Lactic acid fermentation; Corn stover; Simultaneous saccharification and fermentation (SSF); Pediococcus acidilactici DQ2; High lactic acid titer

摘要：lactic acid bacterium with high tolerance of temperature and lignocellulose derived inhibitor was isolated and characterized as Pediococcus acidilactici DQ2. The strain used in the simultaneous saccharification and fermentation (SSF) for high titer lactic acid production at the high solids loading of corn stover. Corn stover was pretreated using the dry sulphuric acid pretreatment, followed by a biological detoxification to remove the inhibitors produced in the pretreatment. The bioreactor with a novel helical impeller was used to the SSF operation of the pretreated and biodetoxified corn stover. The results show that a typical SSF operation at 48 degrees C, pH 5.5, and near 30% (w/w) solids loading in both 5 and 50 L bioreactors was demonstrated. The lactic acid titer, yield, and productivity reached 101.9 g/L, 77.2%, and 1.06 g/L/h, respectively. The result provided a practical process option for cellulosic lactic acid production using virgin agriculture lignocellulose residues. (C) 2012 Elsevier Ltd. All rights reserved.