文献类型：期刊文献

英文题名：Label-free chlorine and nitrogen-doped fluorescent carbon dots for target imaging of lysosomes in living cells

作者：Chang, Shuai[1,2];Chen, Bin Bin[1,2];Lv, Jian[1,2];Fodjo, Essy Kouadio[1,2];Qian, Ruo Can[1,2];Li, Da Wei[1,2]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai Key Lab Funct Mat Chem, Key Lab Adv Mat,Joint Int Lab Precis Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2020

卷号：187

期号：8

外文期刊名：MICROCHIMICA ACTA

收录：;EI(收录号：20242616401267);WOS:【SCI-EXPANDED(收录号:WOS:000552001600001)】；

基金：We are grateful for the financial support from the National Natural Science Foundation of China (Grant Nos. 21788102, 21777041, 21974046, 21977031), the Shanghai Science and Technology Committee (Grant Nos. 17520750100, 19391901700, 19520744000, 19ZR1472300), the National Science and Technology Major Project of China (2018ZX10302205), and the Fundamental Research Funds for the Central Universities (222201714008).

语种：英文

外文关键词：Carbon dots; Chlorine and nitrogen doping; Lysosomes targeting; Long-term imaging; Living cells

摘要：Lysosomes with a single-layered membrane structure are mainly involved in the scavenging of foreign substances and play an important role in maintaining normal physiological functions of living cells. In this work, near-neutrally charged fluorescent carbon dots (CDs) were prepared with lipophilicity through a facile one-pot hydrothermal carbonization of chloranil and triethylenetetramine at 160 degrees C for 3 h. The as-obtained CDs are proved to have good photostability, low cost, and excellent biocompatibility. Importantly, the as-prepared CDs with high quantum yield of 30.8% show excitation-dependent emission with great stability, and thus, they can be well used for the long-term target imaging of lysosomes in living cells without further modification. Meanwhile, the CDs can quickly enter into the lysosomes within 30 min, and the green fluorescence (FL) of CDs reaches the plateau when incubated for 60 min. By comparing the fluorescent intensity, the information about distribution and amount of lysosomes in different cells can be obtained. The proposed CD-based strategy demonstrates great promise for label-free target imaging of lysosomes in living cells. The near-neutral carbon dots (CDs) with lipophilicity are used as label-free fluorescent nanoprobes for the long-term imaging of lysosomes in living cells.