文献类型：期刊文献

英文题名：Palmitoyl copper peptide and acetyl tyrosine complex enhances melanin production in both A375 and B16 cell lines

作者：Hong, Minhua[2];Gui, Yingyue[1];Xu, Jiayao[1];Zhao, Xianglong[2];Jiang, Chunyang[2];Zhao, Jian[1];Xin, Xiujuan[1];Liu, Dan[2];Tang, Xiaolin[2];Tang, Rong[2];An, Faliang[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Peptide Biotechnol Co Ltd, 1777 Hualong Rd, Shanghai 201705, Peoples R China;[3]Marine Biomed Sci & Technol Innovat Platform Lin G, 4,Lane 218,Haiji Sixth Rd, Shanghai 201306, Peoples R China

年份：2025

卷号：742

外文期刊名：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001373230300001)】；

基金：This work was financially supported by Shanghai Peptide Biotechnology Co. Ltd.

语种：英文

外文关键词：Tyrosinase enzyme activity; Melanin; Copper peptide; Melanogenic agent

摘要：Copper peptide, a low molecular weight peptide composed of glycyl-L-histidyl-L-lysine-copper, possesses antiaging, anti-inflammatory, and wound healing properties. In this study, we investigated the effects of a combination agent CP-AcT, composed of palmitoyl copper peptide (pal-GHK-Cu) and acetyl tyrosine (N-Acetyl-Ltyrosine), on melanin production in the human malignant melanoma cell line A375 and the mouse melanoma cell line B16. Firstly, the cytotoxicity of CP-AcT at various concentrations (0-8 mu g/mL) on HaCat, HFF, A375, and B16 cells was evaluated. Subsequently, the effects of the CP-AcT on tyrosinase activity both extracellular and intracellularly, as well as on melanin production in two melanoma cell lines, were evaluated under conditions that did not compromise cell viability. Additionally, quantitative gene plex (QGP) combined with branched DNA (bDNA) technology was used to analyze the effects of CP-AcT on the expression of melanin-related genes in A375 cells, with a focus on five specific genes. Finally, the effects of the CP-AcT on the expression of three proteins involved in the biosynthesis pathway of melanin: tyrosinase (TYR), dopachrome tautomerase (DCT), and endothelin 3 (EDN3) were analyzed. The results indicate that the complex CP-AcT effectively promotes melanin production in both types of melanoma cells.