文献类型：期刊文献

英文题名：Stereocomplementary Bioreduction of β-Ketonitrile without Ethylated Byproduct

作者：Xu, Guo-Chao[1];Yu, Hui-Lei[1];Zhang, Zhi-Jun[1];Xu, Jian-He[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biocatalysis & Synthet Biotechnol, Shanghai 200237, Peoples R China

年份：2013

卷号：15

期号：21

起止页码：5408

外文期刊名：ORGANIC LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000326615100004)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 21276082), Ministry of Science and Technology, P.R. China (Nos. 2011CB710800 and 2011AA02A210), China National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204), and Huo Yingdong Education Foundation.

语种：英文

摘要：alpha-Ethylation is competing with the biocatalytic reduction of aromatic beta-ketonitriles in a whole-cell system. Use of two newly mined robust and stereo-complementary carbonyl reductases in a biphasic system has completely eliminated the competing byproduct For the first time, both enantiomers of fluoroxetine precursors were obtained at 0.5 M with >99% ee and excellent chemoselectivity, without addition of any external cofactors.