文献类型：期刊文献

英文题名：Metagenomics reveals microbial community differences lead to differential nitrate production in anammox reactors with differing nitrogen loading rates

作者：Li, Wei[1,2,3,4];Zhuang, Jin-long[1,2];Zhou, Yuan-yuan[1,2];Meng, Fan-gang[4];Kang, Da[5];Zheng, Ping[5];Shapleigh, James P.[6];Liu, Yong-di[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Resources & Environm Engn, Natl Engn Lab Ind Wastewater Treatment, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai, Peoples R China;[3]Shanghai Inst Pollut Control & Ecol Secur, Shanghai, Peoples R China;[4]Sun Yat Sen Univ, Guangdong Prov Key Lab Environm Pollut Control &, Guangzhou, Guangdong, Peoples R China;[5]Zhejiang Univ, Coll Environm & Resource Sci, Dept Environm Engn, Hangzhou, Peoples R China;[6]Cornell Univ, Dept Microbiol, Ithaca, NY USA

年份：2020

卷号：169

外文期刊名：WATER RESEARCH

收录：;EI(收录号：20194607682644);WOS:【SCI-EXPANDED(收录号:WOS:000509632100073)】；

基金：This work was supported by National Natural Science Foundation of China (51808223, 51578240), Fundamental Research Funds for the Central Universities (222201814053, 222201817009) and Research Fund Program of Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology (2018K16). The authors thank Christina Ferousi for reading an earlier version of the manuscript.

语种：英文

外文关键词：Anammox; Nitrate accumulation; Nitrite oxidoreductase; Metagenomics

摘要：Nitrate production during anammox can decrease total nitrogen removal efficiency, which will negatively impact its usefulness for the removal of nitrogen from waste streams. However, neither the performance characteristics nor physiological shifts associated with nitrate accumulation in anammox reactors under different nitrogen loading rates (NLRB) is well understood. Consequently, these parameters were studied in a lower NLR anammox reactor, termed R1, producing higher than expected levels of nitrate and compared with a higher NLR reactor, termed R2, showing no excess nitrate production. While both reactors showed high NH4+-N removal efficiencies (>90%), the total nitrogen removal efficiency (<60%) was much lower in R1 due to higher nitrate production. Metagenomic analysis found that the number of reads derived from anammox bacteria were significantly higher in R2. Another notable trend in reads occurrence was the relatively higher levels of reads from genes predicted to be nitrite oxidoreductases (nxr) in R1. Binning yielded 27 high quality draft genomes from the two reactors. Analysis of bin occurrence found that R1 showing both a decrease in anammox bacteria and an unexpected increase in nxr. In-situ assays confirmed that R1 had higher rates of nitrite oxidation to nitrate and suggested that it was not solely due to obligate NOB, but other nxr-containing bacteria are important contributors as well. Our results demonstrate that nitrate accumulation can be a serious operational concern for the application of anammox technology to low-strength wastewater treatment and provide insight into the community changes leading to this outcome. (C) 2019 Elsevier Ltd. All rights reserved.