文献类型：期刊文献

英文题名：Identification and characterization of an androgen-responsive Kap promoter enhancer located in the intron II region of human angiotensinogen gene

作者：Fan, Li-qiang[1,2];Hardy, Dianne O.[2];Catterall, James F.[2];Zhao, Jian[1];Li, Su-xia[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Populat Council, Ctr Biomed Res, New York, NY 10021 USA

年份：2010

卷号：119

期号：3-5

起止页码：135

外文期刊名：JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000277095300006)】；

基金：The androgen-responsive promoter pPB(-285/+32)-luc, the androgen receptor expression vector pSG5rAR and glucocorticoid receptor expression vector pSG5-hGR were kindly provided by Jorma Palvimo. University of Helsinki, Finland supported by Science and Technology Commission of Shanghai, Shanghai Pujiang Program 06PJ14024; NIH grant DK069312.

语种：英文

外文关键词：Androgen; Glucocorticoid; Enhancer; Gene regulation; hAGT; Kap promoter

摘要：Tiansgenic expression of the human angiotensinogen (HAGT) gene directed by the mouse kidney androgen-regulated protein (Kap)gene promoter is proximal tubule cell-specific and androgen-regulated in vivo The same Kap promoter fragment did not support similar regulation of other genes, but a transgene based on the original chimeric KAP-hAGT construct successfully directed NHE3 to kidney, suggesting that sequences within the HAGT gene fragment of the construct contributed to the regulation of its expression in vivo. In the present study, androgen-responsive regulatory sequences in the HAGT gene portions of the transgene were examined in transfectecl renal cells A 14-kb enhancer between exons 2 and 3 was identified that increased the basal expression of Kap promoter 1 5- to 2-fold, its induction by dihydrotestosterone (DHT) 2- to 3-fold and its induction by dexamethasone (Dex) 4- to 5-fold Sequence analysis revealed two potential hormone-responsive elements Mutational assays and electrophoretic mobility shift assay showed one of these elements was androgen-specific These findings may influence future strategies for the design of inducible, cell-specific transgenes (C) 2010 Elsevier Ltd All rights reserved