文献类型：期刊文献

英文题名：Enhancement of phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5 using a novel strategy - shaking and extra-light supplementation approach

作者：Li, Xu[1];Wang, Yong-Hong[1];Zhang, Si-Liang[1];Chu, Ju[1];Zhang, Ming[1];Huang, Ming-Zhi[1];Zhuang, Ying-Ping[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2009

卷号：34

期号：24

起止页码：9677

外文期刊名：INTERNATIONAL JOURNAL OF HYDROGEN ENERGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000272856400005)】；

基金：This study was financially supported by Hi-Tech Research and Development Program (863 Program, 2006AA05Z105), National Basic Research Program of China (973 Program, 2007CB714303) and the National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204).

语种：英文

外文关键词：Photo-hydrogen production; Rhodobacter sphaeroides; Light intensity; Hydrogen production rate; Light attenuation kinetics

摘要：Biohydrogen has gained attention due to its potential as a sustainable alternative to conventional methods for hydrogen production. In this study, the effect of light intensity as well as cultivation method (standing- and shaking-culture) on the cell growth and hydrogen production of Rhodobacter sphaeroides ZX-5 were investigated in 38-ml anaerobic photobioreactor with RCVBN medium. Thus, a novel shaking and extra-light supplementation (SELS) approach was developed to enhance the phototrophic H-2 production by R. sphaeroides ZX-5 using malate as the sole carbon source. The optimum illumination condition for shaking-culture by strain ZX-5 increased to 7000-8000 lux, markedly higher than that for standing- culture (4000-5000 lux). Under shaking and elevated illumination (7000-8000 lux), the culture was effective in promoting photo-H-2 production, resulting in a 59% and 56% increase of the maximum and average hydrogen production rate, respectively, in comparison with the culture under standing and 4000-5000 lux conditions. The highest hydrogen-producing rate of 165.9 ml H-2/l h was observed under the application of SELS approach. To our knowledge, this record is currently the highest hydrogen production rate of non-immobilized purple non-sulphur (PNS) bacteria. This optimal performance of photo-H-2 production using SELS approach is a favorable choice of sustainable and economically feasible strategy to improve phototrophic H-2 production efficiency. (C) 2009 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.