文献类型：期刊文献

英文题名：Biocatalytic resolution of glycidyl aryl ethers by Trichosporon loubierii: Cell/substrate ratio influences the optical purity of (R)-epoxides

作者：Xu, Yi[1]; Xu, Jian-He[1]; Pan, Jiang[1]; Tang, Yan-Fa[1]

机构：[1] Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

年份：2004

卷号：26

期号：15

起止页码：1217

外文期刊名：Biotechnology Letters

收录：EI(收录号：2005299218898)

语种：英文

外文关键词：Biocatalysts - Cells - Substrates

摘要：Glycidyl aryl ethers (1a-1e) were resolved by using lyophilized cells of Trichosporon loubierii ECU1040 having epoxide hydrolase activity. The activity and enantioselectivity depended on the structure of the aryl group. Different cell/substrate ratios also influenced the optical purity of remaining substrate. An additional stability test of the whole-cell enzyme suggests that rapid deactivation of the epoxide hydrolase was the potential reason. (R)-Epoxides were prepared in gram amounts with optical purity of 87%-99% ee. ? 2004 Kluwer Academic Publishers.