文献类型：期刊文献

英文题名：Dual-Channel Fluorescent Probe for the Simultaneous Monitoring of Peroxynitrite and Adenosine-5′-triphosphate in Cellular Applications

作者：Wu, Luling[1,2,3,4];Liu, Jihong[1];Tian, Xue[4];Groleau, Robin R.[4];Feng, Beidou[5];Yang, Yonggang[5];Sedgwick, Adam C.[6];Han, Hai-Hao[8,9];Wang, Yang[2,3];Wang, Han-Min[7];Huang, Fang[1];Bull, Steven D.[4];Zhang, Hua[5];Huang, Chusen[2,3];Zang, Yi[7];Li, Jia[7];He, Xiao-Peng[8,9];Li, Ping[1];Tang, Bo[1];James, Tony D.[1,4,5];Sessler, Jonathan L.[6]

机构：[1]Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci, Inst Biomed Sci,Minist Educ,Key Lab Mol & Nano Pr, Jinan 250014, Peoples R China;[2]Shanghai Normal Univ, Dept Chem, Educ Minist, Shanghai Key Lab Rare Earth Funct Mat,Key Lab Res, Shanghai 200234, Peoples R China;[3]Shanghai Normal Univ, Dept Chem, Shanghai Municipal Educ Comm Key Lab Mol Imaging, Shanghai 200234, Peoples R China;[4]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England;[5]Henan Normal Univ, Sch Phys, Xinxiang 453007, Henan, Peoples R China;[6]Univ Texas Austin, Dept Chem, Austin, TX 78712 USA;[7]Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China;[8]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[9]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Joint Int Res Lab Precis Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2022

卷号：144

期号：1

起止页码：174

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20220111415088);WOS:【SCI-EXPANDED(收录号:WOS:000736110100001)】；

基金：L.W. wishes to thank China Scholarship Council and the University of Bath for supporting his Ph.D. in the UK. R.R.G. wishes to thank the EPSRC Centre for Doctoral Training in Catalysis (EP/L016443/1). We would like to thank the EPSRC and the University of Bath for funding. T.D.J. wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University, for support (2020ZD01). P.L. and B.T. thank the National Natural Science Foundation of China (21927811, 22074083), the Key Research and Development Program of Shandong Province (2018YFJH0502), and the National Science Foundation of Shandong Province of China (ZR2020ZD17). C.H. thanks the National Natural Science Foundation of China (Grants 21672150, 21302125), Alexander von Humboldt Foundation (AvH), Shanghai Rising-Star Program (19QA1406400), and the Shanghai Government (18DZ2254200). H.-H.H. would like to thank the Project funded by China Postdoctoral Science Foundation (No. 2020M681196). X.-P.H. thanks the National Natural Science Foundation (No. 91853201), the Shanghai Municipal Science and Technology Major Project (2018SHZDZX03), the National Key Sci-Tech Special Projects of Infection Diseases of China (2018ZX10732202), and the International Cooperation Program of Shanghai Science and Technology Committee (17520750100). H.Z. thanks the National Natural Science Foundation of China (21722501, 11974103), the Zhongyuan High Level Talents Special Support Plan.Science and Technology Innovation Leading Talents (204200510006), and Program for Science Technology Innovation Talents in Universities of Henan Province (21HASTIT019). J.L.S. thanks the Robert A. Welch Foundation (F-0018) for support. NMR spectroscopic and mass spectrometric services were provided by the Material and Chemical Characterisation Facility (MC2) at the University of Bath (http://go.bath.ac.uk/mc2), and we would like to thank Dr. John Lowe for his support with characterization. We are also grateful to Feng-Ming Liu, Yao Li, and Yang Yu from the National Facility for Protein Science in Shanghai (NFPS) for their kind discussions on preliminary bioimaging experiments.

语种：英文

外文关键词：Probes - Phosphates - Adenosinetriphosphate - Sodium compounds

摘要：Changes in adenosine triphosphate (ATP) and peroxynitrite (ONOO-) concentrations have been correlated in a number of diseases including ischemia-reperfusion injury and drug-induced liver injury. Herein, we report the development of a fluorescent probe ATP-LW, which enables the simultaneous detection of ONOO- and ATP. ONOO- selectively oxidizes the boronate pinacol ester of ATP-LW to afford the fluorescent 4-hydroxy-1,8-naphthalimide product NA-OH (lambda(ex) = 450 nm, lambda(em) = 562 nm or lambda(ex) = 488 lambda(em) = 568 nm). In contrast, the binding of ATP to ATP-LW induces the spirolactam ring opening of rhodamine to afford a highly emissive product (lambda(ex )= 520 nm, lambda(em) = 587 nm). Due to the differences in emission between the ONOO- and ATP products, ATP-LW allows ONOO- levels to be monitored in the green channel (lambda(ex) = 488 nm, lambda(em) = 500-575 nm) and ATP concentrations in the red channel (lambda(ex) = 514 nm, lambda(em) = 575-650 nm). The use of ATP-LW as a combined ONOO- and ATP probe was demonstrated using hepatocytes (HL-7702 cells) in cellular imaging experiments. Treatment of HL-7702 cells with oligomycin A (an inhibitor of ATP synthase) resulted in a reduction of signal intensity in the red channel and an increase in that of the green channel as expected for a reduction in ATP concentrations. Similar fluorescence changes were seen in the presence of SIN-1 (an exogenous ONOO- donor).