文献类型：期刊文献

英文题名：Role of Alkaline Serine Protease, Asp, in Vibrio alginolyticus Virulence and Regulation of Its Expression by LuxO-LuxR Regulatory System

作者：Rui Haopeng[1];Liu, Qin[1];Wang, Qiyao[1];Ma, Yue[1];Liu, Huan[1];Shi, Cunbin[2];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China

年份：2009

卷号：19

期号：5

起止页码：431

外文期刊名：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000266447800001)】；

基金：We thank Prof. Debra Milton (Umea University, Sweden) and Dr. Biao Kan (The Chinese Academy of Preventive Medicine, China) for kindly sending strains used for the mutant construction in this study. This work was supported by the National Natural Science Foundation of China (No. U0633004).

语种：英文

外文关键词：Alkaline serine protease; asp; quorum sensing; Vibrio alginolyticus

摘要：The alkaline serine protease asp, which was shown to be a virulence factor of vibrio alginolyticus as a purified protein, was cloned from V. alginolyticus EPGS, a strain recently isolated from moribund Epinephelus coioides in an outbreak of vibriosis in a mariculture farm of Shenzhen. The ash null mutant was constructed by homologous recombination with suicide plasmid pNQ705-1. Compared with the wild-type strain, the asp null mutant exhibited a significant decrease of total extracellular protease activity, and caused a 15-fold decrease in virulence of V. alginolyticus. In our previous study, the luxO and luxR(val) genes from V. alginolyticus MVP01 were cloned and identified, and the luxO-luxR(val) regulatory couple was shown to regulate various genes expression, suggesting that it played a central role in the quorum sensing system of V. alginolyticus. In this study, the regulation of the asp gene was analyzed by using RT-PCR and quantitative real-time PCR methods; we proved that its transcription was greatly induced at the late stage of growth and was regulated by a luxO-luxR(val) regulatory system.