文献类型：期刊文献

英文题名：Fingerprint analysis of Ligusticum chuanxiong using hydrophilic interaction chromatography and reversed-phase liquid chromatography

作者：Jin, Yu[1];Liang, Tu[1];Fu, Qing[1];Xiao, Yuan-Sheng[1];Feng, Jia-Tao[1];Ke, Yan-Xiong[2];Liang, Xin-Miao[1,2]

机构：[1]Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2009

卷号：1216

期号：11

起止页码：2136

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000264226500021)】；

基金：This work was supported by the Knowledge Innovation Program of DICR CAS (K2006A3 and K2006A4). The authors gratefully acknowledge the software support of Professor Yi-Zeng Liang in Central South University, Changsha, China.

语种：英文

外文关键词：Fingerprint analysis; Quality control; Ligusticum chuanxiong; Hydrophilic interaction chromatography; (HILIC); Reversed-phase liquid chromatography; (RPLC); Polar compounds

摘要：Fingerprint analysis is considered one of the most powerful approaches to quality control in traditional Chinese medicines (TCMs). In this study, a binary chromatographic fingerprint analysis was developed using hydrophilic interaction chromatography (HILIC) and reversed-phase liquid chromatography (RPLC) to gain more chemical information about polar compounds and weakly polar compounds. This method was used to construct a chromatographic fingerprint of Ligusticum chuanxiong. The two chromatographic methods demonstrated good precision, reproducibility, and stability, with relative standard deviations of <2% for retention time and 7% for peak area for both HILIC and RPLC separations. Data from the analysis of 14 samples by HILIC and RPLC were processed with similarity analysis, with correlation coefficients and congruence coefficients. This binary fingerprint analysis, using two chromatographic modes, is a powerful tool for characterizing the quality of samples, and can be used for the comprehensive quality control of TCMs. (C) 2008 Elsevier B.V. All rights reserved.