文献类型：期刊文献

英文题名：In situ thiolated alginate hydrogel: Instant formation and its application in hemostasis

作者：Xu, Guanzhe[1];Cheng, Liang[2];Zhang, Qintong[1];Sun, Yunlong[1];Chen, Changlin[1];Xu, Heng[3];Chai, Yimin[2];Lang, Meidong[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Adv Polymer Mat, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China;[2]Shanghai 6 Peoples Hosp, Shanghai, Peoples R China;[3]Collaborat Innovat Ctr Petrochem New Mat, Anqing, Anhui, Peoples R China

年份：2016

卷号：31

期号：5

起止页码：721

外文期刊名：JOURNAL OF BIOMATERIALS APPLICATIONS

收录：;EI(收录号：20164502991052);WOS:【SCI-EXPANDED(收录号:WOS:000386976700009)】；

基金：The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the National Natural Science Foundation of China (No. 21274039) and specialized research fund for the doctoral program of higher education (20130074110007).

语种：英文

外文关键词：Sodium alginate; cysteine methyl ester hydrochloride; in situ hydrogel; wound healing; hemostasis

摘要：An in situ formed hydrogel was synthesized by sodium alginate and cysteine methyl ester, which turned the sodium alginate into thiolated alginate (SA-SH). SA-SH can in situ formed into hydrogel (SA-SS-SA) with a large amount of water through covalent bond in less than 20s. The structure characterization showed that the mechanism of SA-SH gelation was thiol-disulfide transformation. The rheology and cytotoxicity experiments of SA-SS-SA hydrogel were also investigated, which indicated that SA-SS-SA hydrogel had an appropriate mechanical strength as well as an excellent biocompatibility. The SA-SS-SA hydrogel would degrade under certain conditions after a few days and its mechanism was disulfide alkaline reduction. Finally, the hemostatic property of SA-SH was tested by rat tail amputation experiment. The time to hemostasis of rat reduced from 8.26min to 3.24min, which proved that SA-SH had an excellent hemostatic property.