文献类型：期刊文献

中文题名：丁香酚与丁香醛缩二甲醇联合抗氧化作用研究

英文题名：Research on Synergistic Antioxidant Activity of Eugenol and Syringaldehyde Dimethylacetal

作者：方雪祥[1];袁慧慧[1];单艳超[1];蓝闽波[1]

机构：[1]华东理工大学化学与分子工程学院上海市功能性材料化学重点实验室

年份：2020

卷号：41

期号：2

起止页码：21

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;北大核心:【北大核心2017】；

基金：中央高校基本科研业务费专项资金(50321101917022)资助

语种：中文

中文关键词：丁香酚;丁香醛缩二甲醇;协同抗氧化作用;DPPH自由基

外文关键词：eugenol;syringaldehyde dimethylacetal;synergistic antioxidant;DPPH free radical

摘要：本文以解决天然抗氧化剂丁香酚在高使用浓度时促氧化等弊端为目的,采用DPPH·清除法结合等效线分析法、紫外分光光度计等研究丁香酚与丁香醛缩二甲醇协同抗氧化作用,以及采用高效液相和高分辨质谱分析复合抗氧化剂清除DPPH·反应产物和可能的结构。结果表明:丁香酚与丁香醛缩二甲醇以7个比例复配后测得的IC50值在等效线分析图上的落点都在理论加和线下方,试验IC50 mix值均小于理论的IC50 add值(P<0.05),相互作用指数λ值皆小于0.9,表明丁香酚与丁香醛缩二甲醇具有明显的协同抗氧化作用;且最佳协同比例条件下丁香酚-丁香醛缩二甲醇(1∶2)复配物清除DPPH·的能力与其2倍浓度的丁香酚相当。结合反应产物的HPLC谱图分析,推测且分离得到了关键产物:4-((E)-3-(2,6-二甲氧基-4-(二甲氧甲基)苯氧基)烯丙亚基)-2-甲氧基环己-2,5-二烯酮,由此推导出复合抗氧化剂协同作用的反应机制:反应沿着醌类产物消耗的方向进行。
The purpose of this paper was to solve the problem of eugenol on oxidation promotion at high concentration.The interaction of eugenol and syringaldehyde dimethylacetal(SGD)on their antioxidant activity was investigated using 2,2-diphenyl-1-picrylhydrazyl(DPPH)free radical scavenging method,isobolographic analysis,and ultraviolet spectrophotometer.The products of DPPH free radical scavenging reactions of eugenol,SGD and the combined antioxidants were measured by HPLC and high resolution mass spectrum.The results showed that the experimental IC50 value point of seven tested combined antioxidants,eugenol and SGD with different ratios,were all located below the additive line and less than theoretical IC50 value(P<0.05),and their interaction indexλof combination were less than 0.9,which depicted a significant synergistic antioxidant effect.In addition,the eugenol in combined antioxidant with optimum ratio 1∶2 worked almost as twice eugenol used alone in the process of DPPH·scavenging reaction.The key product,4-((E)-3-(2,6-dimethoxy-4-(dimethoxymethyl)phenoxy)allylidene)-2-methoxycyclohexa-2,5-dienone,was speculated by analysing the HPLC chromatogram of products of DPPH free radical scavenging reactions and its high resolution mass spectrum.Consequently,the possible synergistic antioxidant mechanism of the combined antioxidants against DPPH·were deduced that the direction of reaction was quinonoids consuming.