文献类型：期刊文献

英文题名：Enantioseparation of Citalopram by RP-HPLC, Using Sulfobutyl Ether-β-Cyclodextrin as a Chiral Mobile Phase Additive

作者：Peng, Yangfeng[1];He, Quan Sophia[2];Cai, Jiang[1]

机构：[1]E China Univ Sci & Technol, Sch Chem Engn, Shanghai 200237, Peoples R China;[2]Dalhousie Univ, Dept Engn, Fac Agr, Truro, NS B2N 5E3, Canada

年份：2016

卷号：2016

外文期刊名：INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY

收录：;EI(收录号：20214311071929);WOS:【SCI-EXPANDED(收录号:WOS:000368279700001)】；

语种：英文

外文关键词：High performance liquid chromatography - Sodium compounds

摘要：Enantiomeric separation of citalopram (CIT) was developed using a reversed phase HPLC (RP-HPLC) with sulfobutylether-beta-cyclodextrin (SBE-beta-CD) as a chiral mobile phase additive. The effects of the pH value of aqueous buffer, concentration of chiral additive, composition of mobile phase, and column temperature on the enantioseparation of CIT were investigated on the Hedera ODS-2 C-18 column (250 mmx 4.6 mmx 5.0 um). A satisfactory resolution was achieved at 25 degrees C using a mobile phase consisting of a mixture of aqueous buffer (pH of 2.5, 5 mM sodium dihydrogen phosphate, and 12 mM SBE-beta-CD), methanol, and acetonitrile with a volumetric ratio of 21 : 3 : 1 and flow rate of 1.0 mL/min. This analytical method was evaluated by examining the precision (lower than 3.0%), linearity (regression coefficients close to 1), limit of detection (0.070 mu g/mL for (R)-CIT and 0.076 mu g/mL for (S)-CIT), and limit of quantitation (0.235 mu g/mL for (R)-CIT and 0.254 mu g/mL for (S)-CIT).