文献类型：期刊文献

英文题名：Partition of Tea Saponin with a Novel Recyclable Thermo-pH Aqueous Two-Phase Systems

作者：Wei, Yanli[1];Chen, Xi[1];Yang, Ting[1];Wan, Junfen[1];Cao, Xuejun[1]

机构：[1]East China Univ Sci & Technol, Coll Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2021

卷号：193

期号：10

起止页码：3062

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;EI(收录号：20220206674);WOS:【SCI-EXPANDED(收录号:WOS:000651543400001)】；

语种：英文

外文关键词：Aqueous two-phase systems; Partition; Polymer recovery; Tea saponin

摘要：Aqueous two-phase systems (ATPS) have the advantages of environmentally friendly, high mass transfer efficiency, and mild extraction conditions. However, it is difficult to recycle these polymers, which limits the large-scale application of ATPS. In this study, a novel recyclable ATPS was constructed with thermo-responsive polymer P-N and pH-responsive polymer P-ADB4.78 for the partition of tea saponin. P-N represents poly-(N-isopropylacrylamide), and P-ADB4.78 represents poly-(acrylic acid-dimethylamine ethyl methacrylate-butyl methacrylate), where 4.78 in the subscript indicate the isoelectric point of the polymer. The recoveries of P-N and P-ADB4.78 were 95.36% and 93.48%, respectively, after two cycles. Meanwhile, the phase formation mechanism of ATPS was studied by surface tension and low-field nuclear magnetic resonance (LF-NMR). The effects of polymer concentration, pH, temperature, types and concentrations of salt were investigated on tea saponin partition. In the 1.5% (w/v) P-N/3.5% (w/v) P-ADB4.78 ATPS, the optimal partition coefficient (K) of crude tea saponin were 0.15 in the presence of 1.5 mM KCl at pH 7.6 and 25 degrees C while the extraction recovery (ERb) reached 92.13%. The K and ERb of tea saponin from tea seeds were 0.12 and 94.50% with 7.5 mM LiBr at pH 8.0 and 25 degrees C, respectively.